› 8bf8

Electron Microscopy
2.8Å resolution

ISDra2 TnpB in complex with reRNA

Released:
DOI: 10.2210/pdb8bf8/pdb
PDB entry 8bf8 coloured by chain, front view.
PDB entry 8bf8 coloured by chain, side view.
PDB entry 8bf8 coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
TnpB structure reveals minimal functional core of Cas12 nuclease family.
Sasnauskas G, Tamulaitiene G, Druteika G, Carabias A, Silanskas A, Kazlauskas D, Venclovas Č, Montoya G, Karvelis T, Siksnys V
Nature (2023)
PMID: 37020015
Related structures: EMD-16016

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-214966 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-guided DNA endonuclease TnpB Chain: A
Molecule details ›
Chain: A
Length: 408 amino acids
Theoretical weight: 46.48 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7DF80 (Residues: 1-408; Coverage: 100%)
Gene names: DR_0666, DR_0978, DR_1381, DR_1593, DR_1651, DR_1933, DR_2324, orf2, tnpB, tnpC
Sequence domains:
Deinococcus radiodurans R1 chromosome 1 Chain: B
Molecule details ›
Chain: B
Length: 150 nucleotides
Theoretical weight: 48.41 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-16016
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Progress and Prospects of Gene Editing in Pluripotent Stem Cells.
Zhang et al. (2023)
1 more