› 8bid

X-ray diffraction
1.75Å resolution

O-Methyltransferase Plu4890 (mutant H229N) in complex with SAH and AQ-270a

Released:
DOI: 10.2210/pdb8bid/pdb
PDB entry 8bid coloured by chain, front view.
PDB entry 8bid coloured by chain, side view.
PDB entry 8bid coloured by chain, top view.
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Primary publication:
A set of closely related methyltransferases for site-specific tailoring of anthraquinone pigments.
Huber EM, Kreling L, Heinrich AK, Dünnebacke M, Pöthig A, Bode HB, Groll M
Structure (2023)
PMID: 36963398

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227932 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-methyltransferase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 319 amino acids
Theoretical weight: 36.53 KDa
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7MY08 (Residues: 1-318; Coverage: 100%)
Gene name: plu4890
Sequence domains: O-methyltransferase domain

Ligands and Environments

5 bound ligands:
Ligand SAH 4 x SAH
Ligand QPF 4 x QPF
Ligand NA 19 x NA
Ligand CL 4 x CL
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 63.4Å b: 77.67Å c: 167.33Å
α: 90° β: 95.49° γ: 90°
R-values:
R R work R free
0.182 0.18 0.217
Expression system: Escherichia coli BL21(DE3)

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