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O-Methyltransferase Plu4890 (mutant H229N) in complex with SAH and AQ-270a

X-ray diffraction
1.75Å resolution
PDB entry 8bid coloured by chain, front view.
PDB entry 8bid coloured by chain, side view.
PDB entry 8bid coloured by chain, top view.
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: O-methyltransferase domain-containing protein
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Multimeric state: homo dimer
Accessible surface area: 25943.56 Å2
Buried surface area: 7072.35 Å2
Dissociation area: 3,035.82 Å2
Dissociation energy (ΔGdiss): 47.42 kcal/mol
Dissociation entropy (TΔSdiss): 14.35 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-227932
Assembly 2 (preferred)
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Assembly Name: O-methyltransferase domain-containing protein
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Multimeric state: homo dimer
Accessible surface area: 25603.0 Å2
Buried surface area: 9381.76 Å2
Dissociation area: 173.95 Å2
Dissociation energy (ΔGdiss): 20.76 kcal/mol
Dissociation entropy (TΔSdiss): 0.36 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-227932

Macromolecules

O-methyltransferase domain-containing protein
Chains: A, B, C, D
Length: 319 amino acids
Theoretical weight: 36.53 KDa
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7MY08 (Residues: 1-318; Coverage: 100%)
Gene name: plu4890
Pfam: O-methyltransferase domain
InterPro:
O-methyltransferase domain-containing protein in PDB entry 8bid, assembly 2, front view.
O-methyltransferase domain-containing protein in PDB entry 8bid, assembly 2, side view.
O-methyltransferase domain-containing protein in PDB entry 8bid, assembly 2, top view.
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