› 8btg

Electron Microscopy
3.2Å resolution

Cryo-EM structure of the bacterial replication origin opening basal unwinding system

Released:
DOI: 10.2210/pdb8btg/pdb
PDB entry 8btg coloured by chain, front view.
PDB entry 8btg coloured by chain, side view.
PDB entry 8btg coloured by chain, top view.
Source organisms:
Primary publication:
The bacterial replication origin BUS promotes nucleobase capture.
Pelliciari S, Bodet-Lefèvre S, Fenyk S, Stevens D, Winterhalter C, Schramm FD, Pintar S, Burnham DR, Merces G, Richardson TT, Tashiro Y, Hubbard J, Yardimci H, Ilangovan A, Murray H
Nat Commun 14 8339 (2023)
PMID: 38097584
Related structures: EMD-16229

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243660 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Chromosomal replication initiator protein DnaA Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 446 amino acids
Theoretical weight: 50.93 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P05648 (Residues: 1-446; Coverage: 100%)
Gene names: BSU00010, dnaA, dnaH
Sequence domains:
DNA (5'-D(P*AP*CP*TP*TP*AP*TP*CP*CP*AP*CP*AP*AP*AP*TP*CP*CP*AP*CP*AP*GP*GP*CP*CP*C)-3') Chain: X
Molecule details ›
Chain: X
Length: 24 nucleotides
Theoretical weight: 7.23 KDa
Source organism: DNA molecule
Expression system: Not provided
DNA (41-MER) Chain: Y
Molecule details ›
Chain: Y
Length: 41 nucleotides
Theoretical weight: 12.86 KDa
Source organism: DNA molecule
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 7 x MG
Ligand ATP 7 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-16229
Expression systems:
  • Escherichia coli
  • Not provided

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