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Cryo-EM structure of the bacterial replication origin opening basal unwinding system

Electron Microscopy
3.2Å resolution
PDB entry 8btg coloured by chain, front view.
PDB entry 8btg coloured by chain, side view.
PDB entry 8btg coloured by chain, top view.
Source organisms:
Assembly composition:
hetero nonamer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Chromosomal replication initiator protein DnaA and DNA
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Multimeric state: hetero nonamer
Accessible surface area: 116085.53 Å2
Buried surface area: 34992.5 Å2
Dissociation area: 2,619.82 Å2
Dissociation energy (ΔGdiss): 15.15 kcal/mol
Dissociation entropy (TΔSdiss): 15.38 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-243660

Macromolecules

Chromosomal replication initiator protein DnaA
Chains: A, B, C, D, E, F, G
Length: 446 amino acids
Theoretical weight: 50.93 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P05648 (Residues: 1-446; Coverage: 100%)
Gene names: BSU00010, dnaA, dnaH
Pfam:
Chromosomal replication initiator protein DnaA in PDB entry 8btg, assembly 1, front view.
Chromosomal replication initiator protein DnaA in PDB entry 8btg, assembly 1, side view.
Chromosomal replication initiator protein DnaA in PDB entry 8btg, assembly 1, top view.
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DNA chain X
Name: DNA (5'-D(P*AP*CP*TP*TP*AP*TP*CP*CP*AP*CP*AP*AP*AP*TP*CP*CP*AP*CP*AP*GP*GP*CP*CP*C)-3')
Representative chains: X
Source organism: DNA molecule [2853804]
Expression system: Not provided
Length: 24 nucleotides
Theoretical weight: 7.23 KDa
DNA (5'-D(P*AP*CP*TP*TP*AP*TP*CP*CP*AP*CP*AP*AP*AP*TP*CP*CP*AP*CP*AP*GP*GP*CP*CP*C)-3') in PDB entry 8btg, assembly 1, front view.
DNA (5'-D(P*AP*CP*TP*TP*AP*TP*CP*CP*AP*CP*AP*AP*AP*TP*CP*CP*AP*CP*AP*GP*GP*CP*CP*C)-3') in PDB entry 8btg, assembly 1, side view.
DNA (5'-D(P*AP*CP*TP*TP*AP*TP*CP*CP*AP*CP*AP*AP*AP*TP*CP*CP*AP*CP*AP*GP*GP*CP*CP*C)-3') in PDB entry 8btg, assembly 1, top view.
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DNA chain Y
Name: DNA (41-MER)
Representative chains: Y
Source organism: DNA molecule [2853804]
Expression system: Not provided
Length: 41 nucleotides
Theoretical weight: 12.86 KDa
DNA (41-MER) in PDB entry 8btg, assembly 1, front view.
DNA (41-MER) in PDB entry 8btg, assembly 1, side view.
DNA (41-MER) in PDB entry 8btg, assembly 1, top view.
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