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Light-adapted 2.0 Angstrom crystal structure of H132A variant of cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH obtained under aerobic conditions

X-ray diffraction
2Å resolution
PDB entry 8c36 coloured by chain, front view.
PDB entry 8c36 coloured by chain, side view.
PDB entry 8c36 coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Probable transcriptional regulator
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Multimeric state: homo tetramer
Accessible surface area: 29330.86 Å2
Buried surface area: 16567.64 Å2
Dissociation area: 1,516.56 Å2
Dissociation energy (ΔGdiss): 1.48 kcal/mol
Dissociation entropy (TΔSdiss): 13.4 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-181008

Macromolecules

Probable transcriptional regulator
Chains: A, B, C, D
Length: 215 amino acids
Theoretical weight: 23.19 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q746J7 (Residues: 78-285; Coverage: 73%)
Gene name: TT_P0056
Pfam: B12 binding domain
Probable transcriptional regulator in PDB entry 8c36, assembly 1, front view.
Probable transcriptional regulator in PDB entry 8c36, assembly 1, side view.
Probable transcriptional regulator in PDB entry 8c36, assembly 1, top view.
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