› 8c36

X-ray diffraction
2Å resolution

Light-adapted 2.0 Angstrom crystal structure of H132A variant of cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH obtained under aerobic conditions

Released:
DOI: 10.2210/pdb8c36/pdb
PDB entry 8c36 coloured by chain, front view.
PDB entry 8c36 coloured by chain, side view.
PDB entry 8c36 coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Redox driven B12-ligand switch drives CarH photoresponse.
Poddar H, Rios-Santacruz R, Heyes DJ, Shanmugam M, Brookfield A, Johannissen LO, Levy CW, Jeffreys LN, Zhang S, Sakuma M, Colletier JP, Hay S, Schirò G, Weik M, Scrutton NS, Leys D
OpenAccess logo Nat Commun 14 5082 (2023)
PMID: 37604813

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable transcriptional regulator Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 215 amino acids
Theoretical weight: 23.19 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q746J7 (Residues: 78-285; Coverage: 73%)
Gene name: TT_P0056
Sequence domains: B12 binding domain

Ligands and Environments

1 bound ligand:
Ligand B12 4 x B12
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 63.405Å b: 70.664Å c: 203.687Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.218
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Redox driven B12-ligand switch drives CarH photoresponse.
Poddar et al. (2023)