› 8cxa

X-ray diffraction
1.65Å resolution

Crystal Structure of 3-oxoacyl-[acyl-carrier-protein] reductase from Mycobacterium smegmatis with bound NAD

Released:
DOI: 10.2210/pdb8cxa/pdb
PDB entry 8cxa coloured by chain, front view.
PDB entry 8cxa coloured by chain, side view.
PDB entry 8cxa coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 273 amino acids
Theoretical weight: 29.27 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0QVD5 (Residues: 1-251; Coverage: 100%)
Gene name: MSMEG_2536
Sequence domains: Enoyl-(Acyl carrier protein) reductase

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 60.86Å b: 128.78Å c: 60.9Å
α: 90° β: 96.03° γ: 90°
R-values:
R R work R free
0.158 0.157 0.182
Expression system: Escherichia coli BL21(DE3)

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