› 8d6x

Electron Microscopy
3.2Å resolution

Structure of the Mycobacterium tuberculosis 20S proteasome bound to the ATP-bound Mpa ATPase

Released:
DOI: 10.2210/pdb8d6x/pdb
PDB entry 8d6x coloured by chain, front view.
PDB entry 8d6x coloured by chain, side view.
PDB entry 8d6x coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
The β-Grasp Domain of Proteasomal ATPase Mpa Makes Critical Contacts with the Mycobacterium tuberculosis 20S Core Particle to Facilitate Degradation.
Xiao X, Feng X, Yoo JH, Kovach A, Darwin KH, Li H
mSphere e0027422 (2022)
PMID: 35993699
Related structures: EMD-27225

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 41-mer (preferred)
PDBe Complex ID:
PDB-CPX-161588 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Proteasome-associated ATPase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 609 amino acids
Theoretical weight: 67.49 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WQN5 (Residues: 1-609; Coverage: 100%)
Gene names: MTCY261.11c, Rv2115c, mpa
Sequence domains:
Proteasome subunit alpha Chains: G, H, I, J, K, L, M, N, O, k, l, m, n, o
Molecule details ›
Chains: G, H, I, J, K, L, M, N, O, k, l, m, n, o
Length: 248 amino acids
Theoretical weight: 26.91 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHU1 (Residues: 1-248; Coverage: 100%)
Gene names: Rv2109c, prcA
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c
Molecule details ›
Chains: P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c
Length: 291 amino acids
Theoretical weight: 30.33 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHT9 (Residues: 1-291; Coverage: 100%)
Gene names: Rv2110c, prcB
Sequence domains: Proteasome subunit
Proteasome-associated ATPase Chains: d, e, f, g, h, i, j
Molecule details ›
Chains: d, e, f, g, h, i, j
Length: 4 amino acids
Theoretical weight: 480 Da
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WQN5 (Residues: 606-609; Coverage: 1%)
Gene names: MTCY261.11c, Rv2115c, mpa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-27225
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Best practice: setting up and operating a mid-sized cryo-EM facility.
Meng et al. (2023)

1 mention without citation

The β-Grasp Domain of Proteasomal ATPase Mpa Makes Critical Contacts with the Mycobacterium tuberculosis 20S Core Particle to Facilitate Degradation.
Xiao et al. (2022)