› 8d7r

Electron Microscopy
3.9Å resolution

Cryo-EM structure of human CLCF1 signaling complex: model containing the interaction core region

Released:
DOI: 10.2210/pdb8d7r/pdb
PDB entry 8d7r coloured by chain, front view.
PDB entry 8d7r coloured by chain, side view.
PDB entry 8d7r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the assembly of gp130 family cytokine signaling complexes.
Zhou Y, Stevis PE, Cao J, Saotome K, Wu J, Glatman Zaretsky A, Haxhinasto S, Yancopoulos GD, Murphy AJ, Sleeman MW, Olson WC, Franklin MC
OpenAccess logo Sci Adv 9 eade4395 (2023)
PMID: 36930708
Related structures: EMD-27231

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-150896 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Cardiotrophin-like cytokine factor 1 Chain: D
Molecule details ›
Chain: D
Length: 204 amino acids
Theoretical weight: 23.12 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
UniProt:
  • Canonical: Q9UBD9 (Residues: 28-225; Coverage: 100%)
Gene names: BSF3, CLC, CLCF1, NNT1
Sequence domains: Plethodontid receptivity factor PRF
Ciliary neurotrophic factor receptor subunit alpha Chain: C
Molecule details ›
Chain: C
Length: 324 amino acids
Theoretical weight: 36.12 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
UniProt:
  • Canonical: P26992 (Residues: 23-346; Coverage: 93%)
Gene name: CNTFR
Sequence domains: Fibronectin type III domain
Leukemia inhibitory factor receptor Chain: B
Molecule details ›
Chain: B
Length: 817 amino acids
Theoretical weight: 92.83 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P42702 (Residues: 45-833; Coverage: 75%)
Gene name: LIFR
Sequence domains:
Interleukin-6 receptor subunit beta Chain: A
Molecule details ›
Chain: A
Length: 625 amino acids
Theoretical weight: 71.23 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P40189 (Residues: 23-619; Coverage: 67%)
  • Best match: P40189-2 (Residues: 23-324)
Gene name: IL6ST
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Ligand NAG 10 x NAG
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-27231
Expression systems:
  • Mus musculus
  • Cricetulus griseus

Quick links

Citations

4 citation in other articles

Exogenous l-fucose attenuates neuroinflammation induced by lipopolysaccharide.
Xu et al. (2023)
3 more