› 8de9

Electron Microscopy
3.4Å resolution

Cryo-EM structure of the zebrafish two pore domain K+ channel TREK1 (K2P2.1) in DDM/POPE mixed micelles

Released:
DOI: 10.2210/pdb8de9/pdb
PDB entry 8de9 coloured by chain, front view.
PDB entry 8de9 coloured by chain, side view.
PDB entry 8de9 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Membrane phospholipids control gating of the mechanosensitive potassium leak channel TREK1.
Schmidpeter PAM, Petroff JT, Khajoueinejad L, Wague A, Frankfater C, Cheng WWL, Nimigean CM, Riegelhaupt PM
Nat Commun 14 1077 (2023)
PMID: 36841877
Related structures: EMD-27388

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium channel domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 35.56 KDa
Source organism: Danio rerio
Expression system: Komagataella pastoris
UniProt:
  • Canonical: F1QAI2 (Residues: 14-334; Coverage: 75%)
Gene name: kcnk2a
Sequence domains: Ion channel

Ligands and Environments

2 bound ligands:
Ligand PEV 1 x PEV
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-27388
Expression system: Komagataella pastoris

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