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Cryo-EM structure of the zebrafish two pore domain K+ channel TREK1 (K2P2.1) in DDM/POPE mixed micelles

Electron Microscopy
3.4Å resolution
PDB entry 8de9 coloured by chain, front view.
PDB entry 8de9 coloured by chain, side view.
PDB entry 8de9 coloured by chain, top view.
Source organism: Danio rerio
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Potassium channel domain-containing protein
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Multimeric state: homo dimer
Accessible surface area: 27825.03 Å2
Buried surface area: 12780.34 Å2
Dissociation area: 364.65 Å2
Dissociation energy (ΔGdiss): -2.62 kcal/mol
Dissociation entropy (TΔSdiss): 2.54 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-227607

Macromolecules

Potassium channel domain-containing protein
Chains: A, B
Length: 321 amino acids
Theoretical weight: 35.56 KDa
Source organism: Danio rerio
Expression system: Komagataella pastoris
UniProt:
  • Canonical: F1QAI2 (Residues: 14-334; Coverage: 75%)
Gene name: kcnk2a
Pfam: Ion channel
InterPro:
Potassium channel domain-containing protein in PDB entry 8de9, assembly 1, front view.
Potassium channel domain-containing protein in PDB entry 8de9, assembly 1, side view.
Potassium channel domain-containing protein in PDB entry 8de9, assembly 1, top view.
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