› 8dp0

Electron Microscopy
2.96Å resolution

Structure of p110 gamma bound to the Ras inhibitory nanobody NB7

Released:
DOI: 10.2210/pdb8dp0/pdb
PDB entry 8dp0 coloured by chain, front view.
PDB entry 8dp0 coloured by chain, side view.
PDB entry 8dp0 coloured by chain, top view.
Source organisms:
Primary publication:
Allosteric activation or inhibition of PI3Kγ mediated through conformational changes in the p110γ helical domain.
Harris NJ, Jenkins ML, Nam SE, Rathinaswamy MK, Parson MAH, Ranga-Prasad H, Dalwadi U, Moeller BE, Sheeky E, Hansen SD, Yip CK, Burke JE
Elife 12 (2023)
PMID: 37417733
Related structures: EMD-27627

Function and Biology Details

Reactions catalysed: 
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225664 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform Chain: A
Molecule details ›
Chain: A
Length: 1102 amino acids
Theoretical weight: 126.63 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P48736 (Residues: 1-1102; Coverage: 100%)
Gene name: PIK3CG
Sequence domains:
Nanobody NB7 Chain: B
Molecule details ›
Chain: B
Length: 136 amino acids
Theoretical weight: 14.57 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.96Å
Relevant EMDB volumes: EMD-27627
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 citations in other articles

ESAT-6 undergoes self-association at phagosomal pH and an ESAT-6-specific nanobody restricts M. tuberculosis growth in macrophages.
Bates et al. (2024)

2 mentions without citation

Allosteric activation or inhibition of PI3Kγ mediated through conformational changes in the p110γ helical domain.
Harris et al. (2023)
1 more