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Cas7-11 in complex with Csx29

Electron Microscopy
2.95Å resolution
PDB entry 8eex coloured by chain, front view.
PDB entry 8eex coloured by chain, side view.
PDB entry 8eex coloured by chain, top view.
Source organism: Desulfonema ishimotonii
Assembly composition:
hetero trimer (preferred)
Entry contents: 2 distinct polypeptide molecules
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: CHAT domain-containing protein and CRISPR-associated RAMP family protein and RNA
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Multimeric state: hetero trimer
Accessible surface area: 87078.38 Å2
Buried surface area: 19209.94 Å2
Dissociation area: 6,078.92 Å2
Dissociation energy (ΔGdiss): 140.15 kcal/mol
Dissociation entropy (TΔSdiss): 14.95 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-105004

Macromolecules

CRISPR-associated RAMP family protein
Chain: A
Length: 1601 amino acids
Theoretical weight: 183.84 KDa
Source organism: Desulfonema ishimotonii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A401FT36 (Residues: 1-1601; Coverage: 100%)
Gene name: DENIS_1082
Pfam: RAMP superfamily
InterPro: CRISPR type III-associated protein
CRISPR-associated RAMP family protein in PDB entry 8eex, assembly 1, front view.
CRISPR-associated RAMP family protein in PDB entry 8eex, assembly 1, side view.
CRISPR-associated RAMP family protein in PDB entry 8eex, assembly 1, top view.
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CHAT domain-containing protein
Chain: B
Length: 751 amino acids
Theoretical weight: 88.31 KDa
Source organism: Desulfonema ishimotonii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A401FT52 (Residues: 1-751; Coverage: 100%)
Gene name: DENIS_1081
Pfam: CHAT domain
InterPro: CHAT domain
CHAT domain-containing protein in PDB entry 8eex, assembly 1, front view.
CHAT domain-containing protein in PDB entry 8eex, assembly 1, side view.
CHAT domain-containing protein in PDB entry 8eex, assembly 1, top view.
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RNA chain C
Name: crRNA
Representative chains: C
Length: 36 nucleotides
Theoretical weight: 11.5 KDa
crRNA in PDB entry 8eex, assembly 1, front view.
crRNA in PDB entry 8eex, assembly 1, side view.
crRNA in PDB entry 8eex, assembly 1, top view.
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