8eoi

Electron Microscopy
3.4Å resolution

Structure of a human EMC:human Cav1.2 channel complex in GDN detergent

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-216245 (preferred)
Entry contents:
11 distinct polypeptide molecules
Macromolecules (12 distinct):
ER membrane protein complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 973 amino acids
Theoretical weight: 109.6 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8N766 (Residues: 21-993; Coverage: 100%)
Gene names: EMC1, KIAA0090, PSEC0263
Sequence domains:
ER membrane protein complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 291 amino acids
Theoretical weight: 34.25 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15006 (Residues: 3-293; Coverage: 98%)
Gene names: EMC2, KIAA0103, TTC35
Sequence domains: EMC2 TPR-like repeat domain
ER membrane protein complex subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 258 amino acids
Theoretical weight: 29.72 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9P0I2 (Residues: 4-261; Coverage: 99%)
Gene names: EMC3, TMEM111
Sequence domains: Integral membrane protein EMC3/TMCO1-like
ER membrane protein complex subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 101 amino acids
Theoretical weight: 11.43 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8N4V1 (Residues: 3-103; Coverage: 77%)
Gene names: EMC5, MMGT1, TMEM32
Sequence domains: Membrane magnesium transporter
ER membrane protein complex subunit 6 Chain: F
Molecule details ›
Chain: F
Length: 100 amino acids
Theoretical weight: 11.01 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BV81 (Residues: 11-110; Coverage: 91%)
Gene names: EMC6, TMEM93
Sequence domains: EMC6
Endoplasmic reticulum membrane protein complex subunit 7 Chain: G
Molecule details ›
Chain: G
Length: 116 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NPA0 (Residues: 43-158; Coverage: 53%)
Gene names: C11orf3, C15orf24, EMC7, HT022, UNQ905/PRO1926
Sequence domains: ER membrane protein complex subunit 7, beta-sandwich domain
ER membrane protein complex subunit 8 Chain: H
Molecule details ›
Chain: H
Length: 208 amino acids
Theoretical weight: 23.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O43402 (Residues: 3-210; Coverage: 99%)
Gene names: C16orf2, C16orf4, COX4AL, COX4NB, EMC8, FAM158B, NOC4
Sequence domains: Uncharacterised protein family (UPF0172)
ER membrane protein complex subunit 10 Chain: I
Molecule details ›
Chain: I
Length: 157 amino acids
Theoretical weight: 17 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5UCC4 (Residues: 50-206; Coverage: 66%)
Gene names: C19orf63, EMC10, INM02, UNQ764/PRO1556
Sequence domains: ER membrane protein complex subunit 10
Voltage-dependent L-type calcium channel subunit alpha-1C Chain: K
Molecule details ›
Chain: K
Length: 1550 amino acids
Theoretical weight: 176.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13936 (Residues: 109-1658; Coverage: 70%)
Gene names: CACH2, CACN2, CACNA1C, CACNL1A1, CCHL1A1
Sequence domains:
Voltage-dependent L-type calcium channel subunit beta-3 Chain: J
Molecule details ›
Chain: J
Length: 324 amino acids
Theoretical weight: 36.69 KDa
Source organism: Oryctolagus cuniculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P54286 (Residues: 38-361; Coverage: 68%)
Gene names: CACNB3, CACNLB3
Sequence domains:
ER membrane protein complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 170 amino acids
Theoretical weight: 18.79 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5J8M3 (Residues: 14-183; Coverage: 93%)
Gene names: EMC4, HSPC184, PIG17, TMEM85
Sequence domains: Protein of unknown function (DUF1077)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-28376
Expression system: Homo sapiens