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Chromatin localization of nucleophosmin organizes ribosome biogenesis.

<div class='caption-title'>(A) ChIP-seq data showing Fkbp39 and Fkbp41 localization at rDNA with a peak over the 25S rDNA. The y axis on the right show reads per million (rpm) normalizedover background regions (see STAR Methods). The coordinates of the genomic location are shown on top, and the rDNA locus organization is depicted below.</div>
<div class='caption-title'>(A) Cryo-EM maps of Fkbp39 pull-down from fission yeast cells showing the 4 major states of nascent 60S, representing biogenesis intermediates found in the nucleophosmin-rich granular compartment (GC) of the nucleolus. rRNA is shown in gray, ribosomal proteins in green, and ribosome biogenesis factors in blue. All maps are filtered to the corresponding local resolution.</div>
<div class='caption-title'>(A) In vitro binding followed by electrophoresis showing that Fkbp39 dissociates from nucleosomes or from DNA to bind nascent 60S. Increasing amounts of nascent 60S were added to preformed Fkbp39:nucleosome (left) and Fkbp39:DNA complexes (right). Relative molar ratios of each component are indicated on top (Fkbp39 functional unit is a pentamer). Fkbp39:nucleosome and Fkbp39:DNA complexes are visualized on 6% native PA gel, Fkbp39:n60S complexes on 0.8% TBE agarose gel. Red dash lines indicate the position of the nascent 60S. The gels shown are representative experiments of three independent assays.</div>
Ugolini I, Bilokapic S, Ferrolino M, Teague J, Yan Y, Zhou X, Deshmukh A, White M, Kriwacki RW, Halic M
OpenAccess logo Mol Cell (2022)
Related entries: 8esq, 8esr, 8etc, 8etg, 8eth, 8eti, 8etj, 8eug, 8eui, 8eup, 8euy

Cited: 4 times
EuropePMC logo PMID: 36423630

Abstract

Ribosome biogenesis takes place in the nucleolus, a nuclear membrane-less organelle. Although well studied, it remains unknown how nascent ribosomal subunits separate from the central chromatin compartment and move to the outer granular component, where maturation occurs. We find that the Schizosaccharomyces pombe nucleophosmin-like protein Fkbp39 localizes to rDNA sites encoding the 60S subunit rRNA, and this localization contributes to its specific association with nascent 60S subunits. Fkbp39 dissociates from chromatin to bind nascent 60S subunits, causing the latter to partition away from chromatin and from nascent 40S subunits through liquid-liquid phase separation. In vivo, Fkbp39 binding directs the translocation of nascent 60S subunits toward the nucleophosmin-rich granular component. This process increases the efficiency of 60S subunit assembly, facilitating the incorporation of 60S RNA domain III. Thus, chromatin localization determines the specificity of nucleophosmin in sorting nascent ribosomal subunits and coordinates their movement into specialized assembly compartments within the nucleolus.

Articles - 8ev3 mentioned but not cited (1)

  1. Chromatin localization of nucleophosmin organizes ribosome biogenesis. Ugolini I, Bilokapic S, Ferrolino M, Teague J, Yan Y, Zhou X, Deshmukh A, White M, Kriwacki RW, Halic M. Mol Cell 82 4443-4457.e9 (2022)


Reviews citing this publication (1)

  1. Emergent microenvironments of nucleoli. King MR, Ruff KM, Pappu RV. Nucleus 15 2319957 (2024)

Articles citing this publication (2)

  1. Visualizing the nucleoplasmic maturation of human pre-60S ribosomal particles. Zhang Y, Liang X, Luo S, Chen Y, Li Y, Ma C, Li N, Gao N. Cell Res 33 867-878 (2023)
  2. Phase separation-competent FBL promotes early pre-rRNA processing and translation in acute myeloid leukaemia. Yang L, Zhang Z, Jiang P, Kong D, Yu Z, Shi D, Han Y, Chen E, Zheng W, Sun J, Zhao Y, Luo Y, Shi J, Yao H, Huang H, Qian P. Nat Cell Biol (2024)


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