Structure analysis

Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and azithromycin at 2.50A resolution

X-ray diffraction
2.5Å resolution
Assembly composition:
hetero 53-mer (preferred)
Entry contents: 50 distinct polypeptide molecules
3 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 53-mer
PDBe Complex ID: PDB-CPX-221194
Assembly 2
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Multimeric state: hetero 53-mer
PDBe Complex ID: PDB-CPX-221194

Macromolecules

Chains: 1D, 2D
Length: 276 amino acids
Theoretical weight: 30.53 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60405 (Residues: 1-276; Coverage: 100%)
Gene names: TTHA1689, rplB
Pfam:
PDBe-KB: UniProt Coverage View: P60405  
127620406080100120140160180200220240260
 
100200
UniProt
P60405
Chains
Flexibility predictions
Ligand binding sites

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Chains: 1E, 2E
Length: 206 amino acids
Theoretical weight: 22.45 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN8 (Residues: 1-206; Coverage: 100%)
Gene names: TTHA1692, rplC
Pfam: Ribosomal protein L3
PDBe-KB: UniProt Coverage View: Q5SHN8  
120620406080100120140160180200
 
50100150200
UniProt
Q5SHN8
Chains
Ligand binding sites

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Chains: 1F, 2F
Length: 210 amino acids
Theoretical weight: 23.27 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN9 (Residues: 1-210; Coverage: 100%)
Gene names: TTHA1691, rplD
Pfam: Ribosomal protein L4/L1 family
PDBe-KB: UniProt Coverage View: Q5SHN9  
121020406080100120140160180200
 
50100150200
UniProt
Q5SHN9
Chains
Ligand binding sites

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Chains: 1G, 2G
Length: 182 amino acids
Theoretical weight: 21.06 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHQ0 (Residues: 1-182; Coverage: 100%)
Gene names: TTHA1680, rplE
Pfam:
PDBe-KB: UniProt Coverage View: Q5SHQ0  
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Chains: 1H, 2H
Length: 180 amino acids
Theoretical weight: 19.57 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY8 (Residues: 1-180; Coverage: 100%)
Gene names: TTHA1677, rplF
Pfam: Ribosomal protein L6
PDBe-KB: UniProt Coverage View: P0DOY8  
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Chains: 1I, 2I
Length: 148 amino acids
Theoretical weight: 16.42 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SLQ1 (Residues: 1-148; Coverage: 100%)
Gene names: TTHA0242, rplI
Pfam:
PDBe-KB: UniProt Coverage View: Q5SLQ1  
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Chains: 1N, 2N
Length: 140 amino acids
Theoretical weight: 15.93 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60488 (Residues: 1-140; Coverage: 100%)
Gene names: TTHA1465, rplM
Pfam: Ribosomal protein L13
PDBe-KB: UniProt Coverage View: P60488  
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Chains: 1O, 2O
Length: 122 amino acids
Theoretical weight: 13.32 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHP8 (Residues: 1-122; Coverage: 100%)
Gene names: TTHA1682, rplN
Pfam: Ribosomal protein L14p/L23e
PDBe-KB: UniProt Coverage View: Q5SHP8  
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Chains: 1P, 2P
Length: 150 amino acids
Theoretical weight: 16.32 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHQ7 (Residues: 1-150; Coverage: 100%)
Gene names: TTHA1673, rplO
Pfam: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
PDBe-KB: UniProt Coverage View: Q5SHQ7  
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Chains: 1Q, 2Q
Length: 141 amino acids
Theoretical weight: 15.99 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60489 (Residues: 1-141; Coverage: 100%)
Gene names: TTHA1685, rplP
Pfam: Ribosomal protein L16p/L10e
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Chains: 1R, 2R
Length: 118 amino acids
Theoretical weight: 13.75 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q9Z9H5 (Residues: 1-118; Coverage: 100%)
Gene names: TTHA1663, rpl17, rplQ
Pfam: Ribosomal protein L17
PDBe-KB: UniProt Coverage View: Q9Z9H5  
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Chains: 1S, 2S
Length: 112 amino acids
Theoretical weight: 12.64 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHQ4 (Residues: 1-112; Coverage: 100%)
Gene names: TTHA1676, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
PDBe-KB: UniProt Coverage View: Q5SHQ4  
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Chains: 1T, 2T
Length: 146 amino acids
Theoretical weight: 17.19 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60490 (Residues: 1-146; Coverage: 100%)
Gene names: TTHA1031, rplS
Pfam: Ribosomal protein L19
PDBe-KB: UniProt Coverage View: P60490  
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Chains: 1U, 2U
Length: 118 amino acids
Theoretical weight: 13.78 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60491 (Residues: 1-118; Coverage: 100%)
Gene names: TTHA0553, rplT
Pfam: Ribosomal protein L20
PDBe-KB: UniProt Coverage View: P60491  
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Chains: 1V, 2V
Length: 101 amino acids
Theoretical weight: 11.07 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60492 (Residues: 1-101; Coverage: 100%)
Gene names: TTHA1783, rplU
Pfam: Ribosomal prokaryotic L21 protein
PDBe-KB: UniProt Coverage View: P60492  
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Chains: 1W, 2W
Length: 113 amino acids
Theoretical weight: 12.81 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHP3 (Residues: 1-113; Coverage: 100%)
Gene names: TTHA1687, rplV
Pfam: Ribosomal protein L22p/L17e
PDBe-KB: UniProt Coverage View: Q5SHP3  
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Chains: 1X, 2X
Length: 96 amino acids
Theoretical weight: 10.76 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHP0 (Residues: 1-96; Coverage: 100%)
Gene names: TTHA1690, rplW
Pfam: Ribosomal protein L23
PDBe-KB: UniProt Coverage View: Q5SHP0  
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Chains: 1Y, 2Y
Length: 110 amino acids
Theoretical weight: 12.09 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHP9 (Residues: 1-110; Coverage: 100%)
Gene names: TTHA1681, rplX
Pfam:
PDBe-KB: UniProt Coverage View: Q5SHP9  
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Chains: 1Z, 2Z
Length: 206 amino acids
Theoretical weight: 23.24 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHZ1 (Residues: 1-206; Coverage: 100%)
Gene names: TTHA1589, rpl25, rplY, rptL5
Pfam:
PDBe-KB: UniProt Coverage View: Q5SHZ1  
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Chains: 10, 20
Length: 85 amino acids
Theoretical weight: 9.53 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60493 (Residues: 1-85; Coverage: 100%)
Gene names: TTHA1782, rpmA
Pfam: Ribosomal L27 protein
PDBe-KB: UniProt Coverage View: P60493  
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Chains: 11, 21
Length: 98 amino acids
Theoretical weight: 11 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P60494 (Residues: 1-98; Coverage: 100%)
Gene names: TTHA0042, rpmB
Pfam: Ribosomal L28 family
PDBe-KB: UniProt Coverage View: P60494  
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Chains: 12, 22
Length: 72 amino acids
Theoretical weight: 8.67 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHP6 (Residues: 1-72; Coverage: 100%)
Gene names: TTHA1684, rpmC
Pfam: Ribosomal L29 protein
PDBe-KB: UniProt Coverage View: Q5SHP6  
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Chains: 13, 23
Length: 60 amino acids
Theoretical weight: 6.8 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHQ6 (Residues: 1-60; Coverage: 100%)
Gene names: TTHA1674, rpmD
Pfam: Ribosomal protein L30p/L7e
PDBe-KB: UniProt Coverage View: Q5SHQ6  
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Chains: 14, 24
Length: 71 amino acids
Theoretical weight: 8.3 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJE1 (Residues: 1-71; Coverage: 100%)
Gene names: TTHA1073, rpmE
Pfam: Ribosomal protein L31
PDBe-KB: UniProt Coverage View: Q5SJE1  
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Chains: 15, 25
Length: 60 amino acids
Theoretical weight: 6.72 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80339 (Residues: 1-60; Coverage: 100%)
Gene names: TTHA0418, rpl32, rpmF
Pfam: Ribosomal L32p protein family
PDBe-KB: UniProt Coverage View: P80339  
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Chains: 16, 26
Length: 54 amino acids
Theoretical weight: 6.63 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P35871 (Residues: 1-54; Coverage: 100%)
Gene names: TTHA0250, rpl33, rpmG
Pfam: Ribosomal protein L33
PDBe-KB: UniProt Coverage View: P35871  
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Chains: 17, 27
Length: 49 amino acids
Theoretical weight: 6.13 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80340 (Residues: 1-49; Coverage: 100%)
Gene names: TTHA0446, rpl34, rpmH
Pfam: Ribosomal protein L34
PDBe-KB: UniProt Coverage View: P80340  
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Chains: 18, 28
Length: 65 amino acids
Theoretical weight: 7.51 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SKU1 (Residues: 1-65; Coverage: 100%)
Gene names: TTHA0552, rpmI
Pfam: Ribosomal protein L35
PDBe-KB: UniProt Coverage View: Q5SKU1  
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Chains: 19, 29
Length: 37 amino acids
Theoretical weight: 4.44 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHR2 (Residues: 1-37; Coverage: 100%)
Gene names: TTHA1668, rpmJ
Pfam: Ribosomal protein L36
PDBe-KB: UniProt Coverage View: Q5SHR2  
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Chains: 1b, 2b
Length: 256 amino acids
Theoretical weight: 29.32 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80371 (Residues: 1-256; Coverage: 100%)
Gene names: TTHA0861, rps2, rpsB
Pfam: Ribosomal protein S2
PDBe-KB: UniProt Coverage View: P80371  
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Chains: 1c, 2c
Length: 239 amino acids
Theoretical weight: 26.75 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80372 (Residues: 1-239; Coverage: 100%)
Gene names: TTHA1686, rps3, rpsC
Pfam:
PDBe-KB: UniProt Coverage View: P80372  
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Chains: 1d, 2d
Length: 209 amino acids
Theoretical weight: 24.37 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80373 (Residues: 1-209; Coverage: 100%)
Gene names: TTHA1665, rps4, rpsD
Pfam:
PDBe-KB: UniProt Coverage View: P80373  
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Chains: 1e, 2e
Length: 162 amino acids
Theoretical weight: 17.58 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHQ5 (Residues: 1-162; Coverage: 100%)
Gene names: TTHA1675, rpsE
Pfam:
PDBe-KB: UniProt Coverage View: Q5SHQ5  
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Chains: 1f, 2f
Length: 101 amino acids
Theoretical weight: 11.99 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SLP8 (Residues: 1-101; Coverage: 100%)
Gene names: TTHA0245, rpsF
Pfam: Ribosomal protein S6
PDBe-KB: UniProt Coverage View: Q5SLP8  
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Chains: 1g, 2g
Length: 156 amino acids
Theoretical weight: 18.05 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P17291 (Residues: 1-156; Coverage: 100%)
Gene names: TTHA1696, rps7, rpsG
Pfam: Ribosomal protein S7p/S5e
PDBe-KB: UniProt Coverage View: P17291  
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Chains: 1h, 2h
Length: 138 amino acids
Theoretical weight: 15.87 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY9 (Residues: 1-138; Coverage: 100%)
Gene names: TTHA1678, rpsH
Pfam: Ribosomal protein S8
PDBe-KB: UniProt Coverage View: P0DOY9  
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Chains: 1i, 2i
Length: 128 amino acids
Theoretical weight: 14.41 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80374 (Residues: 1-128; Coverage: 100%)
Gene names: TTHA1464, rps9, rpsI
Pfam: Ribosomal protein S9/S16
PDBe-KB: UniProt Coverage View: P80374  
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Chains: 1j, 2j
Length: 105 amino acids
Theoretical weight: 11.95 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN7 (Residues: 1-105; Coverage: 100%)
Gene names: TTHA1693, rpsJ
Pfam: Ribosomal protein S10p/S20e
PDBe-KB: UniProt Coverage View: Q5SHN7  
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Chains: 1k, 2k
Length: 129 amino acids
Theoretical weight: 13.74 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80376 (Residues: 1-129; Coverage: 100%)
Gene names: TTHA1666, rps11, rpsK
Pfam: Ribosomal protein S11
PDBe-KB: UniProt Coverage View: P80376  
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Chains: 1l, 2l
Length: 132 amino acids
Theoretical weight: 14.68 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN3 (Residues: 1-132; Coverage: 100%)
Gene names: TTHA1697, rpsL
Pfam: Ribosomal protein S12/S23
PDBe-KB: UniProt Coverage View: Q5SHN3  
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Chains: 1m, 2m
Length: 126 amino acids
Theoretical weight: 14.34 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80377 (Residues: 1-126; Coverage: 100%)
Gene names: TTHA1667, rps13, rpsM
Pfam: Ribosomal protein S13/S18
PDBe-KB: UniProt Coverage View: P80377  
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Chains: 1n, 2n
Length: 61 amino acids
Theoretical weight: 7.16 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY6 (Residues: 1-61; Coverage: 100%)
Gene names: TTHA1679, rpsN, rpsZ
Pfam: Ribosomal protein S14p/S29e
PDBe-KB: UniProt Coverage View: P0DOY6  
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Chains: 1o, 2o
Length: 89 amino acids
Theoretical weight: 10.58 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJ76 (Residues: 1-89; Coverage: 100%)
Gene names: TTHA1138, rpsO
Pfam: Ribosomal protein S15
PDBe-KB: UniProt Coverage View: Q5SJ76  
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Chains: 1p, 2p
Length: 88 amino acids
Theoretical weight: 10.41 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJH3 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA1035, rpsP
Pfam: Ribosomal protein S16
PDBe-KB: UniProt Coverage View: Q5SJH3  
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Chains: 1q, 2q
Length: 105 amino acids
Theoretical weight: 12.33 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY7 (Residues: 1-105; Coverage: 100%)
Gene names: TTHA1683, rpsQ
Pfam: Ribosomal protein S17
PDBe-KB: UniProt Coverage View: P0DOY7  
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Chains: 1r, 2r
Length: 88 amino acids
Theoretical weight: 10.26 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SLQ0 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA0243, rpsR
Pfam: Ribosomal protein S18
PDBe-KB: UniProt Coverage View: Q5SLQ0  
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Chains: 1s, 2s
Length: 93 amino acids
Theoretical weight: 10.61 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHP2 (Residues: 1-93; Coverage: 100%)
Gene names: TTHA1688, rpsS
Pfam: Ribosomal protein S19
PDBe-KB: UniProt Coverage View: Q5SHP2  
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Chains: 1t, 2t
Length: 106 amino acids
Theoretical weight: 11.74 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80380 (Residues: 1-106; Coverage: 100%)
Gene names: TTHA1397, rps20, rpsT
Pfam: Ribosomal protein S20
PDBe-KB: UniProt Coverage View: P80380  
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Chains: 1u, 2u
Length: 27 amino acids
Theoretical weight: 3.35 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SIH3 (Residues: 1-27; Coverage: 100%)
Gene names: TTHA1396, rpsU
Pfam: 30S ribosomal protein Thx
PDBe-KB: UniProt Coverage View: Q5SIH3  
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Chains: 1y, 2y
Length: 113 amino acids
Theoretical weight: 12.8 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AD49 (Residues: 1-113; Coverage: 100%)
Gene names: JW2578, b2597, raiA, yfiA
Pfam: Sigma 54 modulation protein / S30EA ribosomal protein
PDBe-KB: UniProt Coverage View: P0AD49  
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Name: 23S ribosomal RNA
Representative chains: 1A, 2A
Length: 2915 nucleotides
Theoretical weight: 948.02 KDa
Rfam: Bacterial large subunit ribosomal RNA
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Name: 5S ribosomal RNA
Representative chains: 1B, 2B
Length: 121 nucleotides
Theoretical weight: 39.19 KDa
Rfam: 5S ribosomal RNA
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Name: 16S ribosomal RNA
Representative chains: 1a, 2a
Length: 1521 nucleotides
Theoretical weight: 493.86 KDa
Rfam: Bacterial small subunit ribosomal RNA
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