› 8fef

Electron Microscopy
2.71Å resolution

Structure of Mce1 transporter from Mycobacterium smegmatis (Map0)

Released:
DOI: 10.2210/pdb8fef/pdb
PDB entry 8fef coloured by chain, front view.
PDB entry 8fef coloured by chain, side view.
PDB entry 8fef coloured by chain, top view.
Source organisms:
Primary publication:
Structure of an endogenous mycobacterial MCE lipid transporter.
Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC
Nature (2023)
PMID: 37495693
Related structures: EMD-29025

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-227044 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Virulence factor Mce family protein Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 43.94 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QNR2 (Residues: 1-409; Coverage: 100%)
Gene name: MSMEG_0134
Sequence domains:
Virulence factor Mce family protein Chain: B
Molecule details ›
Chain: B
Length: 343 amino acids
Theoretical weight: 37.47 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QNR3 (Residues: 1-343; Coverage: 100%)
Gene name: MSMEG_0135
Sequence domains:
Virulence factor Mce family protein, putative Chain: C
Virulence factor mce family protein Chain: D
Virulence factor Mce family protein Chain: E
Mce-family protein mce1f Chain: F
ABC transporter domain-containing protein; Green fluorescent protein Chains: G, H
ABC transporter permease Chain: I
ABC-transporter integral membrane protein Chain: J

Ligands and Environments

1 bound ligand:
Ligand UNL 31 x UNL
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.71Å
Relevant EMDB volumes: EMD-29025

Quick links

Citations

4 review citations

Flipping the switch: dynamic modulation of membrane transporter activity in bacteria.
Elston et al. (2023)
3 more

1 mention without citation

Protein target highlights in CASP15: Analysis of models by structure providers.
Alexander et al. (2023)

Experimental raw data

Links to raw experimental data available for this entry are listed below

Electron microscopy data related to PDB entry 8fef found at the Electron Microscopy Public Image Archive (EMPIAR)

Data DOI: 10.6019/EMPIAR-11343
Datasets: 2
Total size: 20900Gb
EMPIAR thumbnail