› 8ffs

Electron Microscopy
2.96Å resolution

Erythromycin bound Klebsiella pneumoniae AcrB multidrug efflux pump

Released:
DOI: 10.2210/pdb8ffs/pdb
PDB entry 8ffs coloured by chain, front view.
PDB entry 8ffs coloured by chain, side view.
PDB entry 8ffs coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Cryo-EM Structures of the Klebsiella pneumoniae AcrB Multidrug Efflux Pump.
Zhang Z, Morgan CE, Bonomo RA, Yu EW
mBio e0065923 (2023)
PMID: 37067435
Related structures: EMD-29055

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-215265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Efflux pump membrane transporter Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1048 amino acids
Theoretical weight: 113.4 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: W9B4M6 (Residues: 1-1048; Coverage: 100%)
Gene names: B5L96_25060, B6I68_14245, BANRA_03961, BL124_00005200, EAO17_20125, G4V31_01305, GJD52_19690, GJJ08_020700, GJJ18_13925, IMY55_19950, KPZU09_34270, LS45_03030, NCTC5052_00390, QIG75_10355, SAMEA104216642_04010, SAMEA3499874_02421, SAMEA3499901_01083, SAMEA3512100_02852, SAMEA3515122_00719, SAMEA3538424_01568, SAMEA3538658_03115, SAMEA3538828_00630, SAMEA3649591_01590, SAMEA3649615_01319, SAMEA3727706_02433, SAMEA4364603_02893, SAMEA4370542_02341, SAMEA4873597_02682, SAMEA4873632_02671, VKR_03842, acrB, acrB_1, acrB_2, acrB_3, acrB_4
Sequence domains: AcrB/AcrD/AcrF family

Ligands and Environments

1 bound ligand:
Ligand ERY 1 x ERY
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.96Å
Relevant EMDB volumes: EMD-29055
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Antimicrobial Resistance: Two-Component Regulatory Systems and Multidrug Efflux Pumps.
De Gaetano et al. (2023)
2 more

1 mention without citation

Cryo-EM Structures of the Klebsiella pneumoniae AcrB Multidrug Efflux Pump.
Zhang et al. (2023)