› 8fho

Electron Microscopy
2.95Å resolution

Cryo-EM structure of human NCC (class 1)

Released:
DOI: 10.2210/pdb8fho/pdb
PDB entry 8fho coloured by chain, front view.
PDB entry 8fho coloured by chain, side view.
PDB entry 8fho coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Structure and thiazide inhibition mechanism of the human Na-Cl cotransporter.
Fan M, Zhang J, Lee CL, Zhang J, Feng L
Nature (2023)
PMID: 36792826
Related structures: EMD-29097

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Solute carrier family 12 member 3 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1092 amino acids
Theoretical weight: 120.37 KDa
Source organism: Danio rerio
Expression system: Homo sapiens
UniProt:
  • Canonical: P55017 (Residues: 39-130, 131-1021; Coverage: 95%)
Gene names: NCC, SLC12A3, TSC
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 2 x ATP
Ligand XZF 2 x XZF
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.95Å
Relevant EMDB volumes: EMD-29097
Expression system: Homo sapiens

Quick links

Citations

2 review citations

The role of SLC12A family of cation-chloride cotransporters and drug discovery methodologies.
Zhang et al. (2023)
1 more

1 mention without citation

Structure and thiazide inhibition mechanism of the human Na-Cl cotransporter.
Fan et al. (2023)