Structure analysis

Human nuclear pre-60S ribosomal subunit (State I1)

Electron Microscopy
2.67Å resolution
Source organism: Homo sapiens
Assembly composition:
hetero 65-mer (preferred)
Entry contents: 59 distinct polypeptide molecules
4 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Multimeric state: hetero 65-mer
Accessible surface area: 964852.74 Å2
Buried surface area: 648159.07 Å2
Dissociation area: 951.46 Å2
Dissociation energy (ΔGdiss): 9.56 kcal/mol
Dissociation entropy (TΔSdiss): 8.98 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-221148

Macromolecules

Chain: BA
Length: 165 amino acids
Theoretical weight: 17.85 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P30050 (Residues: 1-165; Coverage: 100%)
Gene name: RPL12
Pfam:

Search similar proteins

Chain: BB
Length: 217 amino acids
Theoretical weight: 24.88 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62906 (Residues: 1-217; Coverage: 100%)
Gene names: NEDD6, RPL10A
Pfam: Ribosomal protein L1p/L10e family

Search similar proteins

Chain: BD
Length: 734 amino acids
Theoretical weight: 83.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y4W2 (Residues: 1-734; Coverage: 100%)
Gene names: LAS1L, MSTP060
Pfam: Las1-like

Search similar proteins

Chain: L5
Length: 178 amino acids
Theoretical weight: 20.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62913 (Residues: 1-178; Coverage: 100%)
Gene name: RPL11
Pfam:

Search similar proteins

Chain: L6
Length: 211 amino acids
Theoretical weight: 24.32 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P26373 (Residues: 1-211; Coverage: 100%)
Gene names: BBC1, OK/SW-cl.46, RPL13
Pfam: Ribosomal protein L13e

Search similar proteins

Chain: L7
Length: 203 amino acids
Theoretical weight: 23.63 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P40429 (Residues: 1-203; Coverage: 100%)
Gene name: RPL13A
Pfam: Ribosomal protein L13

Search similar proteins

Chain: L8
Length: 215 amino acids
Theoretical weight: 23.49 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P50914 (Residues: 1-215; Coverage: 100%)
Gene name: RPL14
Pfam: Ribosomal protein L14

Search similar proteins

Chain: L9
Length: 204 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61313 (Residues: 1-204; Coverage: 100%)
Gene names: EC45, RPL15, TCBAP0781
Pfam: Ribosomal L15

Search similar proteins

Chain: LA
Length: 184 amino acids
Theoretical weight: 21.44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18621 (Residues: 1-184; Coverage: 100%)
Gene name: RPL17
Pfam: Ribosomal protein L22p/L17e

Search similar proteins

Chain: LB
Length: 188 amino acids
Theoretical weight: 21.69 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q07020 (Residues: 1-188; Coverage: 100%)
Gene name: RPL18
Pfam: Ribosomal protein 60S L18 and 50S L18e

Search similar proteins

Chain: LC
Length: 176 amino acids
Theoretical weight: 20.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q02543 (Residues: 1-176; Coverage: 100%)
Gene name: RPL18A
Pfam: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A

Search similar proteins

Chain: LD
Length: 196 amino acids
Theoretical weight: 23.54 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P84098 (Residues: 1-196; Coverage: 100%)
Gene name: RPL19
Pfam: Ribosomal protein L19e

Search similar proteins

Chain: LE
Length: 160 amino acids
Theoretical weight: 18.61 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46778 (Residues: 1-160; Coverage: 100%)
Gene name: RPL21
Pfam: Ribosomal protein L21e

Search similar proteins

Chain: LF
Length: 128 amino acids
Theoretical weight: 14.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P35268 (Residues: 1-128; Coverage: 100%)
Gene name: RPL22
Pfam: Ribosomal L22e protein family

Search similar proteins

Chain: LG
Length: 140 amino acids
Theoretical weight: 14.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62829 (Residues: 1-140; Coverage: 100%)
Gene name: RPL23
Pfam: Ribosomal protein L14p/L23e

Search similar proteins

Chain: LH
Length: 156 amino acids
Theoretical weight: 17.74 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62750 (Residues: 1-156; Coverage: 100%)
Gene name: RPL23A
Pfam:

Search similar proteins

Chain: LI
Length: 145 amino acids
Theoretical weight: 17.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61254 (Residues: 1-145; Coverage: 100%)
Gene name: RPL26
Pfam:

Search similar proteins

Chain: LJ
Length: 136 amino acids
Theoretical weight: 15.84 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61353 (Residues: 1-136; Coverage: 100%)
Gene name: RPL27
Pfam:

Search similar proteins

Chain: LK
Length: 148 amino acids
Theoretical weight: 16.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46776 (Residues: 1-148; Coverage: 100%)
Gene name: RPL27A
Pfam: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A

Search similar proteins

Chain: LL
Length: 137 amino acids
Theoretical weight: 15.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46779 (Residues: 1-137; Coverage: 100%)
Gene name: RPL28
Pfam: Ribosomal L28e protein family

Search similar proteins

Chain: LN
Length: 403 amino acids
Theoretical weight: 46.22 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P39023 (Residues: 1-403; Coverage: 100%)
Gene names: OK/SW-cl.32, RPL3
Pfam: Ribosomal protein L3

Search similar proteins

Chain: LO
Length: 115 amino acids
Theoretical weight: 12.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62888 (Residues: 1-115; Coverage: 100%)
Gene name: RPL30
Pfam: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

Search similar proteins

Chain: LP
Length: 125 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62899 (Residues: 1-125; Coverage: 100%)
Gene name: RPL31
Pfam: Ribosomal protein L31e

Search similar proteins

Chain: LQ
Length: 135 amino acids
Theoretical weight: 15.9 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62910 (Residues: 1-135; Coverage: 100%)
Gene names: PP9932, RPL32
Pfam: Ribosomal protein L32

Search similar proteins

Chain: LR
Length: 117 amino acids
Theoretical weight: 13.33 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49207 (Residues: 1-117; Coverage: 100%)
Gene name: RPL34
Pfam: Ribosomal protein L34e

Search similar proteins

Chain: LS
Length: 123 amino acids
Theoretical weight: 14.59 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P42766 (Residues: 1-123; Coverage: 100%)
Gene name: RPL35
Pfam: Ribosomal L29 protein

Search similar proteins

Chain: LT
Length: 110 amino acids
Theoretical weight: 12.56 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18077 (Residues: 1-110; Coverage: 100%)
Gene names: GIG33, RPL35A
Pfam: Ribosomal protein L35Ae

Search similar proteins

Chain: LU
Length: 105 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y3U8 (Residues: 1-105; Coverage: 100%)
Gene name: RPL36
Pfam: Ribosomal protein L36e

Search similar proteins

Chain: LW
Length: 97 amino acids
Theoretical weight: 11.11 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61927 (Residues: 1-97; Coverage: 100%)
Gene name: RPL37
Pfam: Ribosomal protein L37e

Search similar proteins

Chain: LX
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61513 (Residues: 1-92; Coverage: 100%)
Gene name: RPL37A
Pfam: Ribosomal L37ae protein family

Search similar proteins

Chain: LY
Length: 70 amino acids
Theoretical weight: 8.24 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P63173 (Residues: 1-70; Coverage: 100%)
Gene name: RPL38
Pfam: Ribosomal L38e protein family

Search similar proteins

Chain: LZ
Length: 51 amino acids
Theoretical weight: 6.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62891 (Residues: 1-51; Coverage: 100%)
Gene name: RPL39
Pfam: Ribosomal L39 protein

Search similar proteins

Chain: NB
Length: 549 amino acids
Theoretical weight: 62.1 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BVP2 (Residues: 1-549; Coverage: 100%)
Gene names: E2IG3, GNL3, NS
Pfam:

Search similar proteins

Chain: NC
Length: 731 amino acids
Theoretical weight: 83.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13823 (Residues: 1-731; Coverage: 100%)
Gene names: GNL2, NGP1
Pfam:

Search similar proteins

Chain: NF
Length: 260 amino acids
Theoretical weight: 30.14 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O95478 (Residues: 1-260; Coverage: 100%)
Gene names: HUSSY-29, NSA2, TINP1
Pfam: Ribosomal protein S8e

Search similar proteins

Chain: NJ
Length: 485 amino acids
Theoretical weight: 53.39 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NVX2 (Residues: 1-485; Coverage: 100%)
Gene names: HUSSY-07, NLE1
Pfam:

Search similar proteins

Chain: NK
Length: 129 amino acids
Theoretical weight: 15.27 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BRT6 (Residues: 1-129; Coverage: 100%)
Gene names: C12orf31, LLPH, cPERP-G
Pfam: LLP homolog

Search similar proteins

Chain: NL
Length: 478 amino acids
Theoretical weight: 54.5 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NZM5 (Residues: 1-478; Coverage: 100%)
Gene names: GLT, GLTSCR2, NOP53, PICT1
Pfam: Nop53 (60S ribosomal biogenesis)

Search similar proteins

Chain: NP
Length: 134 amino acids
Theoretical weight: 15.23 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00488 (Residues: 1-134; Coverage: 100%)
Gene names: ZNF593, ZT86
Pfam: Zinc-finger double-stranded RNA-binding

Search similar proteins

Chain: NT
Length: 687 amino acids
Theoretical weight: 80.01 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NVU7 (Residues: 1-687; Coverage: 100%)
Gene names: NUC130, SDAD1
Pfam:

Search similar proteins

Chain: NU
Length: 929 amino acids
Theoretical weight: 105.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NXF1 (Residues: 1-929; Coverage: 100%)
Gene names: L18, Nbla10363, TEX10
Pfam: Rix1 complex component involved in 60S ribosome maturation

Search similar proteins

Chains: NV, NW
Length: 432 amino acids
Theoretical weight: 47.46 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BV38 (Residues: 1-432; Coverage: 100%)
Gene name: WDR18
Pfam:

Search similar proteins

Chains: NX, NY
Length: 1130 amino acids
Theoretical weight: 119.79 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q8IZL8 (Residues: 1-1130; Coverage: 100%)
Gene names: HMX3, MNAR, PELP1
Pfam:

Search similar proteins

Chain: NZ
Length: 360 amino acids
Theoretical weight: 40.31 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9H6F5 (Residues: 1-360; Coverage: 100%)
Gene names: CCDC86, CYCLON
Pfam: Cgr1 family

Search similar proteins

Chain: SA
Length: 427 amino acids
Theoretical weight: 47.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P36578 (Residues: 1-427; Coverage: 100%)
Gene names: RPL1, RPL4
Pfam:

Search similar proteins

Chain: SB
Length: 297 amino acids
Theoretical weight: 34.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46777 (Residues: 1-297; Coverage: 100%)
Gene names: MSTP030, RPL5
Pfam:

Search similar proteins

Chain: SC
Length: 288 amino acids
Theoretical weight: 32.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q02878 (Residues: 1-288; Coverage: 100%)
Gene names: RPL6, TXREB1
Pfam:

Search similar proteins

Chain: SD
Length: 248 amino acids
Theoretical weight: 29.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18124 (Residues: 1-248; Coverage: 100%)
Gene name: RPL7
Pfam:

Search similar proteins

Chain: SE
Length: 266 amino acids
Theoretical weight: 30.06 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62424 (Residues: 1-266; Coverage: 100%)
Gene names: RPL7A, SURF-3, SURF3
Pfam: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

Search similar proteins

Chain: SF
Length: 257 amino acids
Theoretical weight: 28.09 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62917 (Residues: 1-257; Coverage: 100%)
Gene name: RPL8
Pfam:

Search similar proteins

Chain: SG
Length: 192 amino acids
Theoretical weight: 21.9 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P32969 (Residues: 1-192; Coverage: 100%)
Gene names: OK/SW-cl.103, RPL9, RPL9P7, RPL9P8, RPL9P9
Pfam: Ribosomal protein L6

Search similar proteins

Chain: SH
Length: 293 amino acids
Theoretical weight: 34.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BYG3 (Residues: 1-293; Coverage: 100%)
Gene names: MKI67IP, NIFK, NOPP34
Pfam:

Search similar proteins

Chain: SI
Length: 255 amino acids
Theoretical weight: 29.73 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q6DKI1 (Residues: 1-255; Coverage: 100%)
Gene name: RPL7L1
Pfam:

Search similar proteins

Chain: SK
Length: 245 amino acids
Theoretical weight: 26.62 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P56537 (Residues: 1-245; Coverage: 100%)
Gene names: EIF3A, EIF6, ITGB4BP, OK/SW-cl.27
Pfam: eIF-6 family

Search similar proteins

Chain: SL
Length: 490 amino acids
Theoretical weight: 55.09 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O76021 (Residues: 1-490; Coverage: 100%)
Gene names: CATX11, CSIG, L12, PBK1, RSL1D1
Pfam: Ribosomal protein L1p/L10e family

Search similar proteins

Chain: SM
Length: 588 amino acids
Theoretical weight: 68.11 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00541 (Residues: 1-588; Coverage: 100%)
Gene name: PES1
Pfam:

Search similar proteins

Chain: SQ
Length: 239 amino acids
Theoretical weight: 27.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9UKD2 (Residues: 1-239; Coverage: 100%)
Gene names: C1orf33, MRT4, MRTO4
Pfam:

Search similar proteins

Chain: SR
Length: 634 amino acids
Theoretical weight: 74.11 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BZE4 (Residues: 1-634; Coverage: 100%)
Gene names: CRFG, GTPBP4, NOG1
Pfam:

Search similar proteins

Chain: SV
Length: 163 amino acids
Theoretical weight: 19.67 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9UHA3 (Residues: 1-163; Coverage: 100%)
Gene names: C15orf15, My024, RPL24L, RSL24D1
Pfam: Ribosomal protein L24e

Search similar proteins

Name: 5.8S ribosomal RNA
Representative chains: L1
Length: 157 nucleotides
Theoretical weight: 50.46 KDa
Rfam: 5.8S ribosomal RNA

Search similar RNA

Name: ITS2 rRNA
Representative chains: L2
Length: 1167 nucleotides
Theoretical weight: 376.43 KDa

Search similar RNA

Name: 28S ribosomal RNA
Representative chains: L3
Length: 5070 nucleotides
Theoretical weight: 1641.2 KDa
Rfam: Eukaryotic large subunit ribosomal RNA

Search similar RNA

Name: 5S ribosomal RNA
Representative chains: L4
Length: 121 nucleotides
Theoretical weight: 39 KDa
Rfam: 5S ribosomal RNA

Search similar RNA