› 8fl3

Electron Microscopy
2.53Å resolution

Human nuclear pre-60S ribosomal subunit (State I2)

Released:
DOI: 10.2210/pdb8fl3/pdb
PDB entry 8fl3 coloured by chain, front view.
PDB entry 8fl3 coloured by chain, side view.
PDB entry 8fl3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Principles of human pre-60S biogenesis.
Vanden Broeck A, Klinge S
Science 381 eadh3892 (2023)
PMID: 37410842
Related structures: EMD-29266

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 63-mer (preferred)
PDBe Complex ID:
PDB-CPX-221149 (preferred)
Entry contents:
58 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (61 distinct):
Large ribosomal subunit protein uL11 Chain: BA
Molecule details ›
Chain: BA
Length: 165 amino acids
Theoretical weight: 17.85 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P30050 (Residues: 1-165; Coverage: 100%)
Gene name: RPL12
Sequence domains:
Large ribosomal subunit protein uL1 Chain: BB
Molecule details ›
Chain: BB
Length: 217 amino acids
Theoretical weight: 24.88 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62906 (Residues: 1-217; Coverage: 100%)
Gene names: NEDD6, RPL10A
Sequence domains: Ribosomal protein L1p/L10e family
Ribosomal biogenesis protein LAS1L Chain: BD
Molecule details ›
Chain: BD
Length: 734 amino acids
Theoretical weight: 83.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y4W2 (Residues: 1-734; Coverage: 100%)
Gene names: LAS1L, MSTP060
Sequence domains: Las1-like
Large ribosomal subunit protein uL5 Chain: L5
Molecule details ›
Chain: L5
Length: 178 amino acids
Theoretical weight: 20.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62913 (Residues: 1-178; Coverage: 100%)
Gene name: RPL11
Sequence domains:
Large ribosomal subunit protein eL13 Chain: L6
Molecule details ›
Chain: L6
Length: 211 amino acids
Theoretical weight: 24.32 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P26373 (Residues: 1-211; Coverage: 100%)
Gene names: BBC1, OK/SW-cl.46, RPL13
Sequence domains: Ribosomal protein L13e
Large ribosomal subunit protein uL13 Chain: L7
Molecule details ›
Chain: L7
Length: 203 amino acids
Theoretical weight: 23.63 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P40429 (Residues: 1-203; Coverage: 100%)
Gene name: RPL13A
Sequence domains: Ribosomal protein L13
Large ribosomal subunit protein eL14 Chain: L8
Molecule details ›
Chain: L8
Length: 215 amino acids
Theoretical weight: 23.49 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P50914 (Residues: 1-215; Coverage: 100%)
Gene name: RPL14
Sequence domains: Ribosomal protein L14
Large ribosomal subunit protein eL15 Chain: L9
Molecule details ›
Chain: L9
Length: 204 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61313 (Residues: 1-204; Coverage: 100%)
Gene names: EC45, RPL15, TCBAP0781
Sequence domains: Ribosomal L15
Large ribosomal subunit protein uL22 Chain: LA
Molecule details ›
Chain: LA
Length: 184 amino acids
Theoretical weight: 21.44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18621 (Residues: 1-184; Coverage: 100%)
Gene name: RPL17
Sequence domains: Ribosomal protein L22p/L17e
Large ribosomal subunit protein eL18 Chain: LB
Molecule details ›
Chain: LB
Length: 188 amino acids
Theoretical weight: 21.69 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q07020 (Residues: 1-188; Coverage: 100%)
Gene name: RPL18
Sequence domains: Ribosomal protein 60S L18 and 50S L18e
Large ribosomal subunit protein eL20 Chain: LC
Molecule details ›
Chain: LC
Length: 176 amino acids
Theoretical weight: 20.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q02543 (Residues: 1-176; Coverage: 100%)
Gene name: RPL18A
Sequence domains: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
Large ribosomal subunit protein eL19 Chain: LD
Molecule details ›
Chain: LD
Length: 196 amino acids
Theoretical weight: 23.54 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P84098 (Residues: 1-196; Coverage: 100%)
Gene name: RPL19
Sequence domains: Ribosomal protein L19e
Large ribosomal subunit protein eL21 Chain: LE
Molecule details ›
Chain: LE
Length: 160 amino acids
Theoretical weight: 18.61 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46778 (Residues: 1-160; Coverage: 100%)
Gene name: RPL21
Sequence domains: Ribosomal protein L21e
Large ribosomal subunit protein eL22 Chain: LF
Molecule details ›
Chain: LF
Length: 128 amino acids
Theoretical weight: 14.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P35268 (Residues: 1-128; Coverage: 100%)
Gene name: RPL22
Sequence domains: Ribosomal L22e protein family
Large ribosomal subunit protein uL14 Chain: LG
Molecule details ›
Chain: LG
Length: 140 amino acids
Theoretical weight: 14.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62829 (Residues: 1-140; Coverage: 100%)
Gene name: RPL23
Sequence domains: Ribosomal protein L14p/L23e
Large ribosomal subunit protein uL23 Chain: LH
Molecule details ›
Chain: LH
Length: 156 amino acids
Theoretical weight: 17.74 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62750 (Residues: 1-156; Coverage: 100%)
Gene name: RPL23A
Sequence domains:
Large ribosomal subunit protein uL24 Chain: LI
Molecule details ›
Chain: LI
Length: 145 amino acids
Theoretical weight: 17.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61254 (Residues: 1-145; Coverage: 100%)
Gene name: RPL26
Sequence domains:
Large ribosomal subunit protein eL27 Chain: LJ
Molecule details ›
Chain: LJ
Length: 136 amino acids
Theoretical weight: 15.84 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61353 (Residues: 1-136; Coverage: 100%)
Gene name: RPL27
Sequence domains:
Large ribosomal subunit protein uL15 Chain: LK
Molecule details ›
Chain: LK
Length: 148 amino acids
Theoretical weight: 16.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46776 (Residues: 1-148; Coverage: 100%)
Gene name: RPL27A
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein eL28 Chain: LL
Molecule details ›
Chain: LL
Length: 137 amino acids
Theoretical weight: 15.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46779 (Residues: 1-137; Coverage: 100%)
Gene name: RPL28
Sequence domains: Ribosomal L28e protein family
Large ribosomal subunit protein uL3 Chain: LN
Molecule details ›
Chain: LN
Length: 403 amino acids
Theoretical weight: 46.22 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P39023 (Residues: 1-403; Coverage: 100%)
Gene names: OK/SW-cl.32, RPL3
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein eL30 Chain: LO
Molecule details ›
Chain: LO
Length: 115 amino acids
Theoretical weight: 12.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62888 (Residues: 1-115; Coverage: 100%)
Gene name: RPL30
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Large ribosomal subunit protein eL31 Chain: LP
Molecule details ›
Chain: LP
Length: 125 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62899 (Residues: 1-125; Coverage: 100%)
Gene name: RPL31
Sequence domains: Ribosomal protein L31e
Large ribosomal subunit protein eL32 Chain: LQ
Molecule details ›
Chain: LQ
Length: 135 amino acids
Theoretical weight: 15.9 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62910 (Residues: 1-135; Coverage: 100%)
Gene names: PP9932, RPL32
Sequence domains: Ribosomal protein L32
Large ribosomal subunit protein eL34 Chain: LR
Molecule details ›
Chain: LR
Length: 117 amino acids
Theoretical weight: 13.33 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49207 (Residues: 1-117; Coverage: 100%)
Gene name: RPL34
Sequence domains: Ribosomal protein L34e
Large ribosomal subunit protein uL29 Chain: LS
Molecule details ›
Chain: LS
Length: 123 amino acids
Theoretical weight: 14.59 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P42766 (Residues: 1-123; Coverage: 100%)
Gene name: RPL35
Sequence domains: Ribosomal L29 protein
Large ribosomal subunit protein eL33 Chain: LT
Molecule details ›
Chain: LT
Length: 110 amino acids
Theoretical weight: 12.56 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18077 (Residues: 1-110; Coverage: 100%)
Gene names: GIG33, RPL35A
Sequence domains: Ribosomal protein L35Ae
Large ribosomal subunit protein eL36 Chain: LU
Molecule details ›
Chain: LU
Length: 105 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y3U8 (Residues: 1-105; Coverage: 100%)
Gene name: RPL36
Sequence domains: Ribosomal protein L36e
Large ribosomal subunit protein eL37 Chain: LW
Molecule details ›
Chain: LW
Length: 97 amino acids
Theoretical weight: 11.11 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61927 (Residues: 1-97; Coverage: 100%)
Gene name: RPL37
Sequence domains: Ribosomal protein L37e
Large ribosomal subunit protein eL43 Chain: LX
Molecule details ›
Chain: LX
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61513 (Residues: 1-92; Coverage: 100%)
Gene name: RPL37A
Sequence domains: Ribosomal L37ae protein family
Large ribosomal subunit protein eL38 Chain: LY
Molecule details ›
Chain: LY
Length: 70 amino acids
Theoretical weight: 8.24 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P63173 (Residues: 1-70; Coverage: 100%)
Gene name: RPL38
Sequence domains: Ribosomal L38e protein family
Large ribosomal subunit protein eL39 Chain: LZ
Molecule details ›
Chain: LZ
Length: 51 amino acids
Theoretical weight: 6.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62891 (Residues: 1-51; Coverage: 100%)
Gene name: RPL39
Sequence domains: Ribosomal L39 protein
Guanine nucleotide-binding protein-like 3 Chain: NB
Molecule details ›
Chain: NB
Length: 549 amino acids
Theoretical weight: 62.1 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BVP2 (Residues: 1-549; Coverage: 100%)
Gene names: E2IG3, GNL3, NS
Sequence domains:
Nucleolar GTP-binding protein 2 Chain: NC
Molecule details ›
Chain: NC
Length: 731 amino acids
Theoretical weight: 83.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13823 (Residues: 1-731; Coverage: 100%)
Gene names: GNL2, NGP1
Sequence domains:
Ribosome biogenesis protein NSA2 homolog Chain: NF
Molecule details ›
Chain: NF
Length: 260 amino acids
Theoretical weight: 30.14 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O95478 (Residues: 1-260; Coverage: 100%)
Gene names: HUSSY-29, NSA2, TINP1
Sequence domains: Ribosomal protein S8e
Notchless protein homolog 1 Chain: NJ
Molecule details ›
Chain: NJ
Length: 485 amino acids
Theoretical weight: 53.39 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NVX2 (Residues: 1-485; Coverage: 100%)
Gene names: HUSSY-07, NLE1
Sequence domains:
Protein LLP homolog Chain: NK
Molecule details ›
Chain: NK
Length: 129 amino acids
Theoretical weight: 15.27 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BRT6 (Residues: 1-129; Coverage: 100%)
Gene names: C12orf31, LLPH, cPERP-G
Sequence domains: LLP homolog
Ribosome biogenesis protein NOP53 Chain: NL
Molecule details ›
Chain: NL
Length: 478 amino acids
Theoretical weight: 54.5 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NZM5 (Residues: 1-478; Coverage: 100%)
Gene names: GLT, GLTSCR2, NOP53, PICT1
Sequence domains: Nop53 (60S ribosomal biogenesis)
Zinc finger protein 593 Chain: NP
Molecule details ›
Chain: NP
Length: 134 amino acids
Theoretical weight: 15.23 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00488 (Residues: 1-134; Coverage: 100%)
Gene names: ZNF593, ZT86
Sequence domains: Zinc-finger double-stranded RNA-binding
Protein SDA1 homolog Chain: NT
Molecule details ›
Chain: NT
Length: 687 amino acids
Theoretical weight: 80.01 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NVU7 (Residues: 1-687; Coverage: 100%)
Gene names: NUC130, SDAD1
Sequence domains:
Testis-expressed protein 10 Chain: NU
Molecule details ›
Chain: NU
Length: 929 amino acids
Theoretical weight: 105.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NXF1 (Residues: 1-929; Coverage: 100%)
Gene names: L18, Nbla10363, TEX10
Sequence domains: Rix1 complex component involved in 60S ribosome maturation
WD repeat-containing protein 18 Chains: NV, NW
Molecule details ›
Chains: NV, NW
Length: 432 amino acids
Theoretical weight: 47.46 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BV38 (Residues: 1-432; Coverage: 100%)
Gene name: WDR18
Sequence domains:
Proline-, glutamic acid- and leucine-rich protein 1 Chains: NX, NY
Molecule details ›
Chains: NX, NY
Length: 1130 amino acids
Theoretical weight: 119.79 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q8IZL8 (Residues: 1-1130; Coverage: 100%)
Gene names: HMX3, MNAR, PELP1
Sequence domains:
Coiled-coil domain-containing protein 86 Chain: NZ
Molecule details ›
Chain: NZ
Length: 360 amino acids
Theoretical weight: 40.31 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9H6F5 (Residues: 1-360; Coverage: 100%)
Gene names: CCDC86, CYCLON
Sequence domains: Cgr1 family
Large ribosomal subunit protein uL4 Chain: SA
Molecule details ›
Chain: SA
Length: 427 amino acids
Theoretical weight: 47.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P36578 (Residues: 1-427; Coverage: 100%)
Gene names: RPL1, RPL4
Sequence domains:
Large ribosomal subunit protein uL18 Chain: SB
Molecule details ›
Chain: SB
Length: 297 amino acids
Theoretical weight: 34.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P46777 (Residues: 1-297; Coverage: 100%)
Gene names: MSTP030, RPL5
Sequence domains:
Large ribosomal subunit protein eL6 Chain: SC
Molecule details ›
Chain: SC
Length: 288 amino acids
Theoretical weight: 32.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q02878 (Residues: 1-288; Coverage: 100%)
Gene names: RPL6, TXREB1
Sequence domains:
Large ribosomal subunit protein uL30 Chain: SD
Molecule details ›
Chain: SD
Length: 248 amino acids
Theoretical weight: 29.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18124 (Residues: 1-248; Coverage: 100%)
Gene name: RPL7
Sequence domains:
Large ribosomal subunit protein eL8 Chain: SE
Molecule details ›
Chain: SE
Length: 266 amino acids
Theoretical weight: 30.06 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62424 (Residues: 1-266; Coverage: 100%)
Gene names: RPL7A, SURF-3, SURF3
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Large ribosomal subunit protein uL2 Chain: SF
Molecule details ›
Chain: SF
Length: 257 amino acids
Theoretical weight: 28.09 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62917 (Residues: 1-257; Coverage: 100%)
Gene name: RPL8
Sequence domains:
Large ribosomal subunit protein uL6 Chain: SG
Molecule details ›
Chain: SG
Length: 192 amino acids
Theoretical weight: 21.9 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P32969 (Residues: 1-192; Coverage: 100%)
Gene names: OK/SW-cl.103, RPL9, RPL9P7, RPL9P8, RPL9P9
Sequence domains: Ribosomal protein L6
MKI67 FHA domain-interacting nucleolar phosphoprotein Chain: SH
Molecule details ›
Chain: SH
Length: 293 amino acids
Theoretical weight: 34.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BYG3 (Residues: 1-293; Coverage: 100%)
Gene names: MKI67IP, NIFK, NOPP34
Sequence domains:
Ribosomal protein uL30-like Chain: SI
Molecule details ›
Chain: SI
Length: 255 amino acids
Theoretical weight: 29.73 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q6DKI1 (Residues: 1-255; Coverage: 100%)
Gene name: RPL7L1
Sequence domains:
Eukaryotic translation initiation factor 6 Chain: SK
Molecule details ›
Chain: SK
Length: 245 amino acids
Theoretical weight: 26.62 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P56537 (Residues: 1-245; Coverage: 100%)
Gene names: EIF3A, EIF6, ITGB4BP, OK/SW-cl.27
Sequence domains: eIF-6 family
Pescadillo homolog Chain: SM
Molecule details ›
Chain: SM
Length: 588 amino acids
Theoretical weight: 68.11 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00541 (Residues: 1-588; Coverage: 100%)
Gene name: PES1
Sequence domains:
mRNA turnover protein 4 homolog Chain: SQ
Molecule details ›
Chain: SQ
Length: 239 amino acids
Theoretical weight: 27.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9UKD2 (Residues: 1-239; Coverage: 100%)
Gene names: C1orf33, MRT4, MRTO4
Sequence domains:
GTP-binding protein 4 Chain: SR
Molecule details ›
Chain: SR
Length: 634 amino acids
Theoretical weight: 74.11 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BZE4 (Residues: 1-634; Coverage: 100%)
Gene names: CRFG, GTPBP4, NOG1
Sequence domains:
Probable ribosome biogenesis protein RLP24 Chain: SV
Molecule details ›
Chain: SV
Length: 163 amino acids
Theoretical weight: 19.67 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9UHA3 (Residues: 1-163; Coverage: 100%)
Gene names: C15orf15, My024, RPL24L, RSL24D1
Sequence domains: Ribosomal protein L24e
5.8S ribosomal RNA Chain: L1
Molecule details ›
Chain: L1
Length: 157 nucleotides
Theoretical weight: 50.46 KDa
Sequence domains: 5.8S ribosomal RNA
28S ribosomal RNA Chain: L3
Molecule details ›
Chain: L3
Length: 5070 nucleotides
Theoretical weight: 1641.2 KDa
Sequence domains: Eukaryotic large subunit ribosomal RNA
5S ribosomal RNA Chain: L4
Molecule details ›
Chain: L4
Length: 121 nucleotides
Theoretical weight: 39 KDa
Sequence domains: 5S ribosomal RNA

Ligands and Environments

5 bound ligands:
Ligand MG 100 x MG
Ligand ZN 5 x ZN
Ligand GTP 1 x GTP
Ligand K 2 x K
Ligand GDP 1 x GDP
10 modified residues:
Ligand PSU 61 x PSU
Ligand OMG 22 x OMG
Ligand A2M 20 x A2M
Ligand 1MA 1 x 1MA
Ligand OMC 15 x OMC
Ligand OMU 9 x OMU
Ligand 5MC 2 x 5MC
Ligand 6MZ 1 x 6MZ
Ligand UR3 1 x UR3
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 2.53Å
Relevant EMDB volumes: EMD-29266

Quick links

Citations

2 citation in other articles

Structural insights into coordinating 5S RNP rotation with ITS2 pre-RNA processing during ribosome formation.
Thoms et al. (2023)
1 more