8fl3

Electron Microscopy
2.53Å resolution

Human nuclear pre-60S ribosomal subunit (State I2)

Released:
Source organism: Homo sapiens
Primary publication:
Principles of human pre-60S biogenesis.
Science 381 eadh3892 (2023)
PMID: 37410842
Related structures: EMD-29266

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 63-mer (preferred)
PDBe Complex ID:
PDB-CPX-221149 (preferred)
Entry contents:
58 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (61 distinct):
Large ribosomal subunit protein uL11 Chain: BA
Molecule details ›
Chain: BA
Length: 165 amino acids
Theoretical weight: 17.85 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P30050 (Residues: 1-165; Coverage: 100%)
Gene name: RPL12
Sequence domains:
Large ribosomal subunit protein uL1 Chain: BB
Molecule details ›
Chain: BB
Length: 217 amino acids
Theoretical weight: 24.88 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62906 (Residues: 1-217; Coverage: 100%)
Gene names: NEDD6, RPL10A
Sequence domains: Ribosomal protein L1p/L10e family
Ribosomal biogenesis protein LAS1L Chain: BD
Large ribosomal subunit protein uL5 Chain: L5
Large ribosomal subunit protein eL13 Chain: L6
Large ribosomal subunit protein uL13 Chain: L7
Large ribosomal subunit protein eL14 Chain: L8
Large ribosomal subunit protein eL15 Chain: L9
Large ribosomal subunit protein uL22 Chain: LA
Large ribosomal subunit protein eL18 Chain: LB
Large ribosomal subunit protein eL20 Chain: LC
Large ribosomal subunit protein eL19 Chain: LD
Large ribosomal subunit protein eL21 Chain: LE
Large ribosomal subunit protein eL22 Chain: LF
Large ribosomal subunit protein uL14 Chain: LG
Large ribosomal subunit protein uL23 Chain: LH
Large ribosomal subunit protein uL24 Chain: LI
Large ribosomal subunit protein eL27 Chain: LJ
Large ribosomal subunit protein uL15 Chain: LK
Large ribosomal subunit protein eL28 Chain: LL
Large ribosomal subunit protein uL3 Chain: LN
Large ribosomal subunit protein eL30 Chain: LO
Large ribosomal subunit protein eL31 Chain: LP
Large ribosomal subunit protein eL32 Chain: LQ
Large ribosomal subunit protein eL34 Chain: LR
Large ribosomal subunit protein uL29 Chain: LS
Large ribosomal subunit protein eL33 Chain: LT
Large ribosomal subunit protein eL36 Chain: LU
Large ribosomal subunit protein eL37 Chain: LW
Large ribosomal subunit protein eL43 Chain: LX
Large ribosomal subunit protein eL38 Chain: LY
Large ribosomal subunit protein eL39 Chain: LZ
Guanine nucleotide-binding protein-like 3 Chain: NB
Nucleolar GTP-binding protein 2 Chain: NC
Ribosome biogenesis protein NSA2 homolog Chain: NF
Notchless protein homolog 1 Chain: NJ
Protein LLP homolog Chain: NK
Ribosome biogenesis protein NOP53 Chain: NL
Zinc finger protein 593 Chain: NP
Protein SDA1 homolog Chain: NT
Testis-expressed protein 10 Chain: NU
WD repeat-containing protein 18 Chains: NV, NW
Proline-, glutamic acid- and leucine-rich protein 1 Chains: NX, NY
Coiled-coil domain-containing protein 86 Chain: NZ
Large ribosomal subunit protein uL4 Chain: SA
Large ribosomal subunit protein uL18 Chain: SB
Large ribosomal subunit protein eL6 Chain: SC
Large ribosomal subunit protein uL30 Chain: SD
Large ribosomal subunit protein eL8 Chain: SE
Large ribosomal subunit protein uL2 Chain: SF
Large ribosomal subunit protein uL6 Chain: SG
MKI67 FHA domain-interacting nucleolar phosphoprotein Chain: SH
Ribosomal protein uL30-like Chain: SI
Eukaryotic translation initiation factor 6 Chain: SK
Pescadillo homolog Chain: SM
mRNA turnover protein 4 homolog Chain: SQ
GTP-binding protein 4 Chain: SR
Probable ribosome biogenesis protein RLP24 Chain: SV
5.8S ribosomal RNA Chain: L1
28S ribosomal RNA Chain: L3
5S ribosomal RNA Chain: L4

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.53Å
Relevant EMDB volumes: EMD-29266