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Electron Microscopy
4.6Å resolution

Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex in the RNA synthesis state

Released:
Source organism: Saccharomyces cerevisiae
Related structures: EMD-29349

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-233830 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (6 distinct):
DNA polymerase alpha catalytic subunit A Chain: 1
Molecule details ›
Chain: 1
Length: 1468 amino acids
Theoretical weight: 167.03 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P13382 (Residues: 1-1468; Coverage: 100%)
Gene names: CDC17, N2181, POL1, YNL102W
Sequence domains:
DNA primase small subunit Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 47.76 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P10363 (Residues: 1-409; Coverage: 100%)
Gene names: PRI1, YIB8C, YIR008C
Sequence domains: DNA primase small subunit
DNA primase large subunit Chain: B
Molecule details ›
Chain: B
Length: 528 amino acids
Theoretical weight: 62.35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P20457 (Residues: 1-528; Coverage: 100%)
Gene names: PRI2, YKL045W, YKL258
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit
DNA polymerase alpha subunit B Chain: C
Molecule details ›
Chain: C
Length: 705 amino acids
Theoretical weight: 78.87 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38121 (Residues: 1-705; Coverage: 100%)
Gene names: POL12, YBL035C, YBL0414
Sequence domains:
template DNA Chain: T
Molecule details ›
Chain: T
Length: 12 nucleotides
Theoretical weight: 3.6 KDa
Primer RNA Chain: P
Molecule details ›
Chain: P
Length: 8 nucleotides
Theoretical weight: 2.62 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.6Å
Relevant EMDB volumes: EMD-29349
Expression system: Saccharomyces cerevisiae