› 8fx7

X-ray diffraction
2.2Å resolution

Non-ribosomal PCP-C didomain (ester stabilised leucine) acceptor bound state

Released:
DOI: 10.2210/pdb8fx7/pdb
PDB entry 8fx7 coloured by chain, front view.
PDB entry 8fx7 coloured by chain, side view.
PDB entry 8fx7 coloured by chain, top view.
Source organism: Thermobifida fusca YX
Primary publication:
Not always an innocent bystander: the impact of stabilised phosphopantetheine moieties when studying nonribosomal peptide biosynthesis.
Ho YTC, Kaczmarski JA, Tailhades J, Izoré T, Steer DL, Schittenhelm RB, Tosin M, Jackson CJ, Cryle MJ
Chem Commun (Camb) (2023)
PMID: 37310188

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carrier domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 520 amino acids
Theoretical weight: 56.69 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47NR9 (Residues: 2481-3000; Coverage: 14%)
Gene name: Tfu_1867
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand YCT 2 x YCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 104.333Å b: 104.782Å c: 106.557Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Palliative radiotherapy indications during the COVID-19 pandemic and in future complex logistic settings: the NORMALITY model.
Cellini et al. (2021)