› 8ghn

Electron Microscopy
2.96Å resolution

Composite model of the yeast Hir Complex with Asf1/H3/H4

Released:
DOI: 10.2210/pdb8ghn/pdb
PDB entry 8ghn coloured by chain, front view.
PDB entry 8ghn coloured by chain, side view.
PDB entry 8ghn coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the Hir histone chaperone complex.
Kim HJ, Szurgot MR, van Eeuwen T, Ricketts MD, Basnet P, Zhang AL, Vogt A, Sharmin S, Kaplan CD, Garcia BA, Marmorstein R, Murakami K
Mol Cell (2024)
PMID: 38925115
Related structures: EMD-40037

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-247639 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Protein HIR1 Chains: A, G
Molecule details ›
Chains: A, G
Length: 840 amino acids
Theoretical weight: 93.98 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32479 (Residues: 1-840; Coverage: 100%)
Gene names: HIR1, YBL008W, YBL0318
Sequence domains:
Protein HIR2 Chains: B, C, H, I
Molecule details ›
Chains: B, C, H, I
Length: 875 amino acids
Theoretical weight: 98.55 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32480 (Residues: 1-875; Coverage: 100%)
Gene names: HIR2, OR26.31, YOR038C
Sequence domains: TUP1-like enhancer of split
Histone transcription regulator 3 Chains: D, J
Molecule details ›
Chains: D, J
Length: 1648 amino acids
Theoretical weight: 191.92 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P47171 (Residues: 1-1648; Coverage: 100%)
Gene names: HIR3, HPC1, J2161, YJR140C
Histone promoter control protein 2 Chains: E, F, K, L
Molecule details ›
Chains: E, F, K, L
Length: 625 amino acids
Theoretical weight: 67.88 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q01448 (Residues: 1-625; Coverage: 100%)
Gene names: HPC2, YBR1503, YBR215W
Sequence domains: HPC2 and ubinuclein domain
Histone H3 Chain: M
Molecule details ›
Chain: M
Length: 136 amino acids
Theoretical weight: 15.39 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P61830 (Residues: 1-136; Coverage: 100%)
Gene names: HHT1, HHT2, N2749, SIN2, YBR010W, YBR0201, YNL031C
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chain: N
Molecule details ›
Chain: N
Length: 103 amino acids
Theoretical weight: 11.4 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P02309 (Residues: 1-103; Coverage: 100%)
Gene names: HHF1, HHF2, N2752, YBR009C, YBR0122, YNL030W
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone chaperone Chain: O
Molecule details ›
Chain: O
Length: 279 amino acids
Theoretical weight: 31.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.96Å
Relevant EMDB volumes: EMD-40037
Expression system: Escherichia coli

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