› 8gt3

X-ray diffraction
1.5Å resolution

Crystal structure of human cardiac alpha actin P109A mutant (ADP-Pi state) in complex with fragmin F1 domain

Released:
DOI: 10.2210/pdb8gt3/pdb
PDB entry 8gt3 coloured by chain, front view.
PDB entry 8gt3 coloured by chain, side view.
PDB entry 8gt3 coloured by chain, top view.
Source organisms:
Primary publication:
Mutagenic analysis of actin reveals the mechanism of His161 flipping that triggers ATP hydrolysis.
Iwasa M, Takeda S, Narita A, Maéda Y, Oda T
OpenAccess logo Front Cell Dev Biol 11 1105460 (2023)
PMID: 37009486

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159269 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha cardiac muscle 1 Chain: A
Molecule details ›
Chain: A
Length: 391 amino acids
Theoretical weight: 43.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P68032 (Residues: 3-377; Coverage: 100%)
Gene names: ACTC, ACTC1
Sequence domains: Actin
Gelsolin-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 162 amino acids
Theoretical weight: 18.25 KDa
Source organism: Physarum polycephalum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94707 (Residues: 1-160; Coverage: 43%)
Sequence domains: Gelsolin repeat

Ligands and Environments

5 bound ligands:
Ligand ADP 1 x ADP
Ligand PO4 3 x PO4
Ligand MG 1 x MG
Ligand EDO 4 x EDO
Ligand CA 2 x CA
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: AichiSR BEAMLINE BL2S1
Spacegroup: P212121
Unit cell:
a: 57.171Å b: 91.108Å c: 115.142Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.196
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 mention without citation

Mutagenic analysis of actin reveals the mechanism of His161 flipping that triggers ATP hydrolysis.
Iwasa et al. (2023)