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3 nucleotide-bound V1EG of V/A-ATPase from Thermus thermophilus.

Electron Microscopy
3Å resolution
PDB entry 8gxx coloured by chain, front view.
PDB entry 8gxx coloured by chain, side view.
PDB entry 8gxx coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Assembly composition:
hetero dodecamer (preferred)
Entry contents: 6 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: V-type ATP
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Multimeric state: hetero dodecamer
Accessible surface area: 137781.09 Å2
Buried surface area: 48086.58 Å2
Dissociation area: 2,086.5 Å2
Dissociation energy (ΔGdiss): 2.68 kcal/mol
Dissociation entropy (TΔSdiss): 27.41 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-131512

Macromolecules

V-type ATP synthase alpha chain
Chains: A, B, C
Length: 578 amino acids
Theoretical weight: 63.67 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q56403 (Residues: 1-578; Coverage: 100%)
Gene names: TTHA1273, atpA
Pfam:
InterPro:
V-type ATP synthase alpha chain in PDB entry 8gxx, assembly 1, front view.
V-type ATP synthase alpha chain in PDB entry 8gxx, assembly 1, side view.
V-type ATP synthase alpha chain in PDB entry 8gxx, assembly 1, top view.
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V-type ATP synthase beta chain
Chains: D, E, F
Length: 478 amino acids
Theoretical weight: 53.22 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q56404 (Residues: 1-478; Coverage: 100%)
Gene names: TTHA1272, atpB
Pfam:
InterPro:
V-type ATP synthase beta chain in PDB entry 8gxx, assembly 1, front view.
V-type ATP synthase beta chain in PDB entry 8gxx, assembly 1, side view.
V-type ATP synthase beta chain in PDB entry 8gxx, assembly 1, top view.
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V-type ATP synthase subunit D
Chain: G
Length: 223 amino acids
Theoretical weight: 24.72 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: O87880 (Residues: 1-223; Coverage: 100%)
Gene names: TTHA1271, atpD, vatD
Pfam: ATP synthase subunit D
InterPro: ATPase, V1 complex, subunit D
V-type ATP synthase subunit D in PDB entry 8gxx, assembly 1, front view.
V-type ATP synthase subunit D in PDB entry 8gxx, assembly 1, side view.
V-type ATP synthase subunit D in PDB entry 8gxx, assembly 1, top view.
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V-type ATP synthase subunit F
Chain: H
Length: 104 amino acids
Theoretical weight: 11.29 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P74903 (Residues: 1-104; Coverage: 100%)
Gene names: TTHA1274, atpF, vatF
Pfam: ATP synthase (F/14-kDa) subunit
InterPro:
V-type ATP synthase subunit F in PDB entry 8gxx, assembly 1, front view.
V-type ATP synthase subunit F in PDB entry 8gxx, assembly 1, side view.
V-type ATP synthase subunit F in PDB entry 8gxx, assembly 1, top view.
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V-type ATP synthase, subunit (VAPC-THERM)
Chains: I, K
Length: 120 amino acids
Theoretical weight: 13.17 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SIT5 (Residues: 1-120; Coverage: 100%)
Gene name: TTHA1279
Pfam: Vacuolar (H+)-ATPase G subunit
V-type ATP synthase, subunit (VAPC-THERM) in PDB entry 8gxx, assembly 1, front view.
V-type ATP synthase, subunit (VAPC-THERM) in PDB entry 8gxx, assembly 1, side view.
V-type ATP synthase, subunit (VAPC-THERM) in PDB entry 8gxx, assembly 1, top view.
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V-type ATP synthase subunit E
Chains: J, L
Length: 188 amino acids
Theoretical weight: 20.65 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P74901 (Residues: 1-188; Coverage: 100%)
Gene names: TTHA1276, atpE, vatE
Pfam: ATP synthase (E/31 kDa) subunit
InterPro:
V-type ATP synthase subunit E in PDB entry 8gxx, assembly 1, front view.
V-type ATP synthase subunit E in PDB entry 8gxx, assembly 1, side view.
V-type ATP synthase subunit E in PDB entry 8gxx, assembly 1, top view.
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