› 8h69

Electron Microscopy
3.7Å resolution

Cryo-EM structure of influenza RNA polymerase

Released:
DOI: 10.2210/pdb8h69/pdb
PDB entry 8h69 coloured by chain, front view.
PDB entry 8h69 coloured by chain, side view.
PDB entry 8h69 coloured by chain, top view.
Source organism: Influenza A virus (A/goose/Guangdong/1/1996(H5N1))
Primary publication:
An intermediate state allows influenza polymerase to switch smoothly between transcription and replication cycles.
Li H, Wu Y, Li M, Guo L, Gao Y, Wang Q, Zhang J, Lai Z, Zhang X, Zhu L, Lan P, Rao Z, Liu Y, Liang H
Nat Struct Mol Biol (2023)
PMID: 37488357
Related structures: EMD-34497

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-235963 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (5 distinct):
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 716 amino acids
Theoretical weight: 82.67 KDa
Source organism: Influenza A virus (A/goose/Guangdong/1/1996(H5N1))
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q9Q0U9 (Residues: 1-716; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 757 amino acids
Theoretical weight: 86.49 KDa
Source organism: Influenza A virus (A/goose/Guangdong/1/1996(H5N1))
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q9Q0V0 (Residues: 1-757; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chain: C
Molecule details ›
Chain: C
Length: 759 amino acids
Theoretical weight: 85.95 KDa
Source organism: Influenza A virus (A/goose/Guangdong/1/1996(H5N1))
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q9Q0V1 (Residues: 1-759; Coverage: 100%)
Gene name: PB2
Sequence domains:
RNA (5'-R(*UP*AP*AP*AP*CP*UP*CP*CP*UP*GP*CP*UP*UP*UP*UP*GP*CP*U)-3') Chain: 3
Molecule details ›
Chain: 3
Length: 18 nucleotides
Theoretical weight: 5.61 KDa
RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*AP*GP*UP*UP*UP*U)-3') Chain: 5
Molecule details ›
Chain: 5
Length: 19 nucleotides
Theoretical weight: 6.15 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-34497
Expression system: Insect cell expression vector pTIE1

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