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Cryo-EM structure of influenza RNA polymerase

Electron Microscopy
3.7Å resolution
PDB entry 8h69 coloured by chain, front view.
PDB entry 8h69 coloured by chain, side view.
PDB entry 8h69 coloured by chain, top view.
Source organism: Influenza A virus (A/goose/Guangdong/1/1996(H5N1))
Assembly composition:
hetero pentamer (preferred)
Entry contents: 3 distinct polypeptide molecules
2 distinct RNA molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero pentamer
Accessible surface area: 91056.46 Å2
Buried surface area: 41220.37 Å2
Dissociation area: 2,070.16 Å2
Dissociation energy (ΔGdiss): 43.21 kcal/mol
Dissociation entropy (TΔSdiss): 11.71 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-235963

Macromolecules

Polymerase acidic protein
Chain: A
Length: 716 amino acids
Theoretical weight: 82.67 KDa
Source organism: Influenza A virus (A/goose/Guangdong/1/1996(H5N1))
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q9Q0U9 (Residues: 1-716; Coverage: 100%)
Gene name: PA
Pfam: Influenza RNA-dependent RNA polymerase subunit PA
Polymerase acidic protein in PDB entry 8h69, assembly 1, front view.
Polymerase acidic protein in PDB entry 8h69, assembly 1, side view.
Polymerase acidic protein in PDB entry 8h69, assembly 1, top view.
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RNA-directed RNA polymerase catalytic subunit
Chain: B
Length: 757 amino acids
Theoretical weight: 86.49 KDa
Source organism: Influenza A virus (A/goose/Guangdong/1/1996(H5N1))
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q9Q0V0 (Residues: 1-757; Coverage: 100%)
Gene name: PB1
Pfam: Influenza RNA-dependent RNA polymerase subunit PB1
RNA-directed RNA polymerase catalytic subunit in PDB entry 8h69, assembly 1, front view.
RNA-directed RNA polymerase catalytic subunit in PDB entry 8h69, assembly 1, side view.
RNA-directed RNA polymerase catalytic subunit in PDB entry 8h69, assembly 1, top view.
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Polymerase basic protein 2
Chain: C
Length: 759 amino acids
Theoretical weight: 85.95 KDa
Source organism: Influenza A virus (A/goose/Guangdong/1/1996(H5N1))
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q9Q0V1 (Residues: 1-759; Coverage: 100%)
Gene name: PB2
Pfam:
Polymerase basic protein 2 in PDB entry 8h69, assembly 1, front view.
Polymerase basic protein 2 in PDB entry 8h69, assembly 1, side view.
Polymerase basic protein 2 in PDB entry 8h69, assembly 1, top view.
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RNA chain 3
Name: RNA (5'-R(*UP*AP*AP*AP*CP*UP*CP*CP*UP*GP*CP*UP*UP*UP*UP*GP*CP*U)-3')
Representative chains: 3
Length: 18 nucleotides
Theoretical weight: 5.61 KDa
RNA (5'-R(*UP*AP*AP*AP*CP*UP*CP*CP*UP*GP*CP*UP*UP*UP*UP*GP*CP*U)-3') in PDB entry 8h69, assembly 1, front view.
RNA (5'-R(*UP*AP*AP*AP*CP*UP*CP*CP*UP*GP*CP*UP*UP*UP*UP*GP*CP*U)-3') in PDB entry 8h69, assembly 1, side view.
RNA (5'-R(*UP*AP*AP*AP*CP*UP*CP*CP*UP*GP*CP*UP*UP*UP*UP*GP*CP*U)-3') in PDB entry 8h69, assembly 1, top view.
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RNA chain 5
Name: RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*AP*GP*UP*UP*UP*U)-3')
Representative chains: 5
Length: 19 nucleotides
Theoretical weight: 6.15 KDa
RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*AP*GP*UP*UP*UP*U)-3') in PDB entry 8h69, assembly 1, front view.
RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*AP*GP*UP*UP*UP*U)-3') in PDB entry 8h69, assembly 1, side view.
RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*AP*GP*UP*UP*UP*U)-3') in PDB entry 8h69, assembly 1, top view.
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