› 8h7g

Electron Microscopy
3.7Å resolution

Cryo-EM structure of the human SAGA complex

Released:
DOI: 10.2210/pdb8h7g/pdb
PDB entry 8h7g coloured by chain, front view.
PDB entry 8h7g coloured by chain, side view.
PDB entry 8h7g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of human SAGA transcriptional coactivator complex.
Zhang Y, Yin C, Yin Y, Wei M, Jing W, Peng C, Chen Z, Huang J
OpenAccess logo Cell Discov 8 125 (2022)
PMID: 36414614
Related structures: EMD-34520

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-127399 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Transformation/transcription domain-associated protein Chain: C
Molecule details ›
Chain: C
Length: 3859 amino acids
Theoretical weight: 438.14 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y4A5 (Residues: 1-3859; Coverage: 100%)
Gene names: PAF400, TRRAP
Sequence domains:
Splicing factor 3B subunit 3 Chain: A
Molecule details ›
Chain: A
Length: 1217 amino acids
Theoretical weight: 135.72 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q15393 (Residues: 1-1217; Coverage: 100%)
Gene names: KIAA0017, SAP130, SF3B3
Sequence domains:
Splicing factor 3B subunit 5 Chain: B
Molecule details ›
Chain: B
Length: 86 amino acids
Theoretical weight: 10.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BWJ5 (Residues: 1-86; Coverage: 100%)
Gene names: SF3B10, SF3B5
Sequence domains: Splicing factor 3B subunit 10 (SF3b10)
Transcription factor SPT20 homolog Chain: D
Molecule details ›
Chain: D
Length: 779 amino acids
Theoretical weight: 85.88 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q8NEM7 (Residues: 1-779; Coverage: 100%)
Gene names: C13orf19, FAM48A, FP757, SUPT20H
Sequence domains: Spt20, SEP domain
Transcription initiation protein SPT3 homolog Chain: E
Molecule details ›
Chain: E
Length: 317 amino acids
Theoretical weight: 35.84 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O75486 (Residues: 1-317; Coverage: 100%)
Gene names: SPT3, SUPT3H
Sequence domains: Transcription initiation factor IID, 18kD subunit
Transcriptional adapter 1 Chain: G
Molecule details ›
Chain: G
Length: 374 amino acids
Theoretical weight: 41.8 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96BN2 (Residues: 1-335; Coverage: 100%)
Gene names: TADA1, TADA1L
Sequence domains: Transcriptional regulator of RNA polII, SAGA, subunit
TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L Chain: H
Molecule details ›
Chain: H
Length: 589 amino acids
Theoretical weight: 66.22 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O75529 (Residues: 1-589; Coverage: 100%)
Gene names: PAF65B, TAF5L
Sequence domains:
STAGA complex 65 subunit gamma Chain: I
Molecule details ›
Chain: I
Length: 455 amino acids
Theoretical weight: 50.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O94864 (Residues: 1-414; Coverage: 100%)
Gene names: KIAA0764, SUPT7L
Sequence domains: Bromodomain associated
TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L Chain: K
Molecule details ›
Chain: K
Length: 622 amino acids
Theoretical weight: 67.9 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y6J9 (Residues: 1-622; Coverage: 100%)
Gene names: PAF65A, TAF6L
Sequence domains:
Transcription initiation factor TFIID subunit 9 Chain: M
Molecule details ›
Chain: M
Length: 264 amino acids
Theoretical weight: 29.01 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q16594 (Residues: 1-264; Coverage: 100%)
Gene names: TAF2G, TAF9, TAFII31
Sequence domains: Transcription initiation factor IID, 31kD subunit
Transcription initiation factor TFIID subunit 10 Chain: O
Molecule details ›
Chain: O
Length: 218 amino acids
Theoretical weight: 21.73 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q12962 (Residues: 1-218; Coverage: 100%)
Gene names: TAF10, TAF2A, TAF2H, TAFII30
Sequence domains: Transcription initiation factor TFIID 23-30kDa subunit
Transcription initiation factor TFIID subunit 12 Chain: R
Molecule details ›
Chain: R
Length: 161 amino acids
Theoretical weight: 17.95 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q16514 (Residues: 1-161; Coverage: 100%)
Gene names: TAF12, TAF15, TAF2J, TAFII20
Sequence domains: Transcription initiation factor TFIID subunit A
Unassigned sequence Chain: X
Molecule details ›
Chain: X
Length: 19 amino acids
Theoretical weight: 1.64 KDa
Source organism: Homo sapiens
Ataxin-7 Chain: L
Molecule details ›
Chain: L
Length: 892 amino acids
Theoretical weight: 95.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O15265 (Residues: 1-892; Coverage: 100%)
Gene names: ATXN7, SCA7
Sequence domains: SCA7, zinc-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-34520
Expression system: Homo sapiens

Quick links