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Cell divisome sPG hydrolysis machinery FtsEX-EnvC

Electron Microscopy
3.11Å resolution
PDB entry 8hd0 coloured by chain, front view.
PDB entry 8hd0 coloured by chain, side view.
PDB entry 8hd0 coloured by chain, top view.
Source organism: Escherichia coli K-12
Assembly composition:
hetero pentamer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero pentamer
Accessible surface area: 48827.25 Å2
Buried surface area: 9978.66 Å2
Dissociation area: 2,670.12 Å2
Dissociation energy (ΔGdiss): 2.3 kcal/mol
Dissociation entropy (TΔSdiss): 26.89 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-235047

Macromolecules

Cell division ATP-binding protein FtsE
Chains: A, B
Length: 222 amino acids
Theoretical weight: 24.48 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A9R7 (Residues: 1-222; Coverage: 100%)
Gene names: JW3428, b3463, ftsE
Pfam: ABC transporter
Cell division ATP-binding protein FtsE in PDB entry 8hd0, assembly 1, front view.
Cell division ATP-binding protein FtsE in PDB entry 8hd0, assembly 1, side view.
Cell division ATP-binding protein FtsE in PDB entry 8hd0, assembly 1, top view.
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Cell division protein FtsX
Chains: C, D
Length: 352 amino acids
Theoretical weight: 38.58 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AC30 (Residues: 1-352; Coverage: 100%)
Gene names: JW3427, b3462, ftsS, ftsX
Pfam:
Cell division protein FtsX in PDB entry 8hd0, assembly 1, front view.
Cell division protein FtsX in PDB entry 8hd0, assembly 1, side view.
Cell division protein FtsX in PDB entry 8hd0, assembly 1, top view.
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Murein hydrolase activator EnvC
Chain: E
Length: 419 amino acids
Theoretical weight: 46.66 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37690 (Residues: 1-419; Coverage: 100%)
Gene names: JW5646, b3613, envC, yibP
Pfam: Peptidase family M23
Murein hydrolase activator EnvC in PDB entry 8hd0, assembly 1, front view.
Murein hydrolase activator EnvC in PDB entry 8hd0, assembly 1, side view.
Murein hydrolase activator EnvC in PDB entry 8hd0, assembly 1, top view.
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