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Fine structure and assembly pattern of a minimal myophage Pam3.

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Yang F, Jiang YL, Zhang JT, Zhu J, Du K, Yu RC, Wei ZL, Kong WW, Cui N, Li WF, Chen Y, Li Q, Zhou CZ
OpenAccess logo Proc Natl Acad Sci U S A 120 e2213727120 (2023)
Related entries: 7yfw, 7yfz, 8hdr, 8hdt, 8hdw

Cited: 3 times
EuropePMC logo PMID: 36656854

Abstract

The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail contraction mechanism of myophage remain largely unknown. Here, we present the fine structure of a freshwater Myoviridae cyanophage Pam3, which has an icosahedral capsid of ~680 Å in diameter, connected via a three-section neck to an 840-Å-long contractile tail, ending with a three-module baseplate composed of only six protein components. This simplified baseplate consists of a central hub-spike surrounded by six wedge heterotriplexes, to which twelve tail fibers are covalently attached via disulfide bonds in alternating upward and downward configurations. In vitro reduction assays revealed a putative redox-dependent mechanism of baseplate assembly and tail sheath contraction. These findings establish a minimal myophage that might become a user-friendly chassis phage in synthetic biology.

Articles citing this publication (3)

  1. High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217. Li F, Hou CD, Lokareddy RK, Yang R, Forti F, Briani F, Cingolani G. Nat Commun 14 4052 (2023)
  2. Contractile injection systems facilitate sporogenic differentiation of Streptomyces davawensis through the action of a phage tapemeasure protein-related effector. Nagakubo T, Nishiyama T, Yamamoto T, Nomura N, Toyofuku M. Nat Commun 15 4442 (2024)
  3. Structure of the intact tail machine of Anabaena myophage A-1(L). Yu RC, Yang F, Zhang HY, Hou P, Du K, Zhu J, Cui N, Xu X, Chen Y, Li Q, Zhou CZ. Nat Commun 15 2654 (2024)


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