› 8hlb

Electron Microscopy
3.63Å resolution

Cryo-EM structure of biparatopic antibody Bp109-92 in complex with TNFR2

Released:
DOI: 10.2210/pdb8hlb/pdb
PDB entry 8hlb coloured by chain, front view.
PDB entry 8hlb coloured by chain, side view.
PDB entry 8hlb coloured by chain, top view.
Source organisms:
Primary publication:
Development of a 1:1-binding biparatopic anti-TNFR2 antagonist by reducing signaling activity through epitope selection.
Akiba H, Fujita J, Ise T, Nishiyama K, Miyata T, Kato T, Namba K, Ohno H, Kamada H, Nagata S, Tsumoto K
Commun Biol 6 987 (2023)
PMID: 37758868
Related structures: EMD-34871

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230831 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Maltose/maltodextrin-binding periplasmic protein; Tumor necrosis factor-binding protein 2 Chain: A
Molecule details ›
Chain: A
Length: 550 amino acids
Theoretical weight: 60.45 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P20333 (Residues: 23-200; Coverage: 41%)
  • Canonical: P0AEX9 (Residues: 27-391; Coverage: 99%)
Gene names: JW3994, TNFBR, TNFR2, TNFRSF1B, b4034, malE
Sequence domains:
TR109 heavy chain Chain: B
Molecule details ›
Chain: B
Length: 236 amino acids
Theoretical weight: 25.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
TR109 light chain Chain: C
Molecule details ›
Chain: C
Length: 218 amino acids
Theoretical weight: 23.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
TR92 heavy chain Chain: D
Molecule details ›
Chain: D
Length: 238 amino acids
Theoretical weight: 25.54 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
TR92 light chain Chain: E
Molecule details ›
Chain: E
Length: 214 amino acids
Theoretical weight: 23.59 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.63Å
Relevant EMDB volumes: EMD-34871
Expression system: Homo sapiens

Quick links

Citations

1 mention without citation

Development of a 1:1-binding biparatopic anti-TNFR2 antagonist by reducing signaling activity through epitope selection.
Akiba et al. (2023)