Structure analysis

E. coli 70S ribosome complexed with tRNA_Ile2 bearing L34 and t6A37 in classical state

Electron Microscopy
2.32Å resolution
Source organism: Escherichia coli
Assembly composition:
hetero 55-mer (preferred)
Entry contents: 49 distinct polypeptide molecules
6 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 55-mer
Accessible surface area: 757949.92 Å2
Buried surface area: 318274.91 Å2
Dissociation area: 593.04 Å2
Dissociation energy (ΔGdiss): 0.63 kcal/mol
Dissociation entropy (TΔSdiss): 10.09 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-279467

Macromolecules

Chain: B
Length: 241 amino acids
Theoretical weight: 26.78 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V0 (Residues: 1-241; Coverage: 100%)
Gene names: JW0164, b0169, rpsB
Pfam: Ribosomal protein S2

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Chain: C
Length: 233 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V3 (Residues: 1-233; Coverage: 100%)
Gene names: JW3276, b3314, rpsC
Pfam:

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Chain: D
Length: 206 amino acids
Theoretical weight: 23.51 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V8 (Residues: 1-206; Coverage: 100%)
Gene names: JW3258, b3296, ramA, rpsD
Pfam:

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Chain: E
Length: 167 amino acids
Theoretical weight: 17.63 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W1 (Residues: 1-167; Coverage: 100%)
Gene names: JW3265, b3303, rpsE, spc
Pfam:

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Chain: F
Length: 135 amino acids
Theoretical weight: 15.73 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02358 (Residues: 1-135; Coverage: 100%)
Gene names: JW4158, b4200, rpsF
Pfam: Ribosomal protein S6

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Chain: G
Length: 179 amino acids
Theoretical weight: 20.06 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02359 (Residues: 1-179; Coverage: 100%)
Gene names: JW3303, b3341, rpsG
Pfam: Ribosomal protein S7p/S5e

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Chain: H
Length: 130 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W7 (Residues: 1-130; Coverage: 100%)
Gene names: JW3268, b3306, rpsH
Pfam: Ribosomal protein S8

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Chain: I
Length: 130 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7X3 (Residues: 1-130; Coverage: 100%)
Gene names: JW3199, b3230, rpsI
Pfam: Ribosomal protein S9/S16

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Chain: J
Length: 103 amino acids
Theoretical weight: 11.76 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R5 (Residues: 1-103; Coverage: 100%)
Gene names: JW3283, b3321, nusE, rpsJ
Pfam: Ribosomal protein S10p/S20e

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Chain: K
Length: 129 amino acids
Theoretical weight: 13.87 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R9 (Residues: 1-129; Coverage: 100%)
Gene names: JW3259, b3297, rpsK
Pfam: Ribosomal protein S11

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Chain: L
Length: 124 amino acids
Theoretical weight: 13.81 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 1-124; Coverage: 100%)
Gene names: JW3304, b3342, rpsL, strA
Pfam: Ribosomal protein S12/S23

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Chain: M
Length: 118 amino acids
Theoretical weight: 13.13 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S9 (Residues: 1-118; Coverage: 100%)
Gene names: JW3260, b3298, rpsM
Pfam: Ribosomal protein S13/S18

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Chain: N
Length: 101 amino acids
Theoretical weight: 11.61 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG59 (Residues: 1-101; Coverage: 100%)
Gene names: JW3269, b3307, rpsN
Pfam: Ribosomal protein S14p/S29e

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Chain: O
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 1-89; Coverage: 100%)
Gene names: JW3134, b3165, rpsO, secC
Pfam: Ribosomal protein S15

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Chain: P
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T3 (Residues: 1-82; Coverage: 100%)
Gene names: JW2590, b2609, rpsP
Pfam: Ribosomal protein S16

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Chain: Q
Length: 84 amino acids
Theoretical weight: 9.72 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG63 (Residues: 1-84; Coverage: 100%)
Gene names: JW3273, b3311, neaA, rpsQ
Pfam: Ribosomal protein S17

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Chain: R
Length: 75 amino acids
Theoretical weight: 9.01 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T7 (Residues: 1-75; Coverage: 100%)
Gene names: JW4160, b4202, rpsR
Pfam: Ribosomal protein S18

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Chain: S
Length: 92 amino acids
Theoretical weight: 10.46 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U3 (Residues: 1-92; Coverage: 100%)
Gene names: JW3278, b3316, rpsS
Pfam: Ribosomal protein S19

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Chain: T
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U7 (Residues: 1-87; Coverage: 100%)
Gene names: JW0022, b0023, rpsT
Pfam: Ribosomal protein S20

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Chain: U
Length: 71 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P68679 (Residues: 1-71; Coverage: 100%)
Gene names: JW3037, b3065, rpsU
Pfam: Ribosomal protein S21

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Chain: c
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 1-273; Coverage: 100%)
Gene names: JW3279, b3317, rplB
Pfam:

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Chain: d
Length: 209 amino acids
Theoretical weight: 22.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Pfam: Ribosomal protein L3

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Chain: e
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60723 (Residues: 1-201; Coverage: 100%)
Gene names: JW3281, b3319, eryA, rplD
Pfam: Ribosomal protein L4/L1 family

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Chain: f
Length: 179 amino acids
Theoretical weight: 20.33 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P62399 (Residues: 1-179; Coverage: 100%)
Gene names: JW3270, b3308, rplE
Pfam:

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Chain: g
Length: 177 amino acids
Theoretical weight: 18.93 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG55 (Residues: 1-177; Coverage: 100%)
Gene names: JW3267, b3305, rplF
Pfam: Ribosomal protein L6

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Chain: h
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R1 (Residues: 1-149; Coverage: 100%)
Gene names: JW4161, b4203, rplI
Pfam:

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Chain: i
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AA10 (Residues: 1-142; Coverage: 100%)
Gene names: JW3200, b3231, rplM
Pfam: Ribosomal protein L13

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Chain: j
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADY3 (Residues: 1-123; Coverage: 100%)
Gene names: JW3272, b3310, rplN
Pfam: Ribosomal protein L14p/L23e

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Chain: k
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02413 (Residues: 1-144; Coverage: 100%)
Gene names: JW3263, b3301, rplO
Pfam: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A

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Chain: l
Length: 136 amino acids
Theoretical weight: 15.33 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADY7 (Residues: 1-136; Coverage: 100%)
Gene names: JW3275, b3313, rplP
Pfam: Ribosomal protein L16p/L10e

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Chain: m
Length: 127 amino acids
Theoretical weight: 14.39 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG44 (Residues: 1-127; Coverage: 100%)
Gene names: JW3256, b3294, rplQ
Pfam: Ribosomal protein L17

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Chain: n
Length: 117 amino acids
Theoretical weight: 12.79 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0C018 (Residues: 1-117; Coverage: 100%)
Gene names: JW3266, b3304, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

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Chain: o
Length: 115 amino acids
Theoretical weight: 13.16 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7K6 (Residues: 1-115; Coverage: 100%)
Gene names: JW2587, b2606, rplS
Pfam: Ribosomal protein L19

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Chain: p
Length: 118 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L3 (Residues: 1-118; Coverage: 100%)
Gene names: JW1706, b1716, pdzA, rplT
Pfam: Ribosomal protein L20

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Chain: q
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG48 (Residues: 1-103; Coverage: 100%)
Gene names: JW3153, b3186, rplU
Pfam: Ribosomal prokaryotic L21 protein

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Chain: r
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P61175 (Residues: 1-110; Coverage: 100%)
Gene names: JW3277, b3315, eryB, rplV
Pfam: Ribosomal protein L22p/L17e

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Chain: s
Length: 100 amino acids
Theoretical weight: 11.22 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ0 (Residues: 1-100; Coverage: 100%)
Gene names: JW3280, b3318, rplW
Pfam: Ribosomal protein L23

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Chain: t
Length: 104 amino acids
Theoretical weight: 11.34 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60624 (Residues: 1-104; Coverage: 100%)
Gene names: JW3271, b3309, rplX
Pfam:

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Chain: u
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P68919 (Residues: 1-94; Coverage: 100%)
Gene names: JW2173, b2185, rplY
Pfam: Ribosomal L25p family

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Chain: v
Length: 85 amino acids
Theoretical weight: 9.15 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L8 (Residues: 1-85; Coverage: 100%)
Gene names: JW3152, b3185, rpmA
Pfam: Ribosomal L27 protein

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Chain: w
Length: 78 amino acids
Theoretical weight: 9.03 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M2 (Residues: 1-78; Coverage: 100%)
Gene names: JW3612, b3637, rpmB
Pfam: Ribosomal L28 family

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Chain: x
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M6 (Residues: 1-63; Coverage: 100%)
Gene names: JW3274, b3312, rpmC
Pfam: Ribosomal L29 protein

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Chain: y
Length: 59 amino acids
Theoretical weight: 6.55 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG51 (Residues: 1-59; Coverage: 100%)
Gene names: JW3264, b3302, rpmD
Pfam: Ribosomal protein L30p/L7e

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Chain: z
Length: 57 amino acids
Theoretical weight: 6.46 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7N4 (Residues: 1-57; Coverage: 100%)
Gene names: JW1075, b1089, rpmF
Pfam: Ribosomal L32p protein family

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Chain: 0
Length: 55 amino acids
Theoretical weight: 6.39 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7N9 (Residues: 1-55; Coverage: 100%)
Gene names: JW3611, b3636, rpmG
Pfam: Ribosomal protein L33

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Chain: 1
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7P5 (Residues: 1-46; Coverage: 100%)
Gene names: JW3680, b3703, rimA, rpmH, ssaF
Pfam: Ribosomal protein L34

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Chain: 2
Length: 65 amino acids
Theoretical weight: 7.31 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7Q1 (Residues: 1-65; Coverage: 100%)
Gene names: JW1707, b1717, rpmI
Pfam: Ribosomal protein L35

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Chain: 3
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7Q6 (Residues: 1-38; Coverage: 100%)
Gene names: JW3261, b3299, rpmJ
Pfam: Ribosomal protein L36

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Chain: 4
Length: 70 amino acids
Theoretical weight: 7.89 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M9 (Residues: 1-70; Coverage: 100%)
Gene names: JW3907, b3936, rpmE
Pfam: Ribosomal protein L31

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Name: 16S ribosomal RNA
Representative chains: A
Length: 1542 nucleotides
Theoretical weight: 499.87 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: 23S ribosomal RNA
Representative chains: a
Length: 2904 nucleotides
Theoretical weight: 941.81 KDa
Rfam: Bacterial large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: b
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Rfam: 5S ribosomal RNA

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Name: P-site tRNA_Glu
Representative chains: V
Length: 76 nucleotides
Theoretical weight: 24.47 KDa
Rfam: tRNA

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Name: Messenger RNA
Representative chains: W
Length: 48 nucleotides
Theoretical weight: 15.58 KDa

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Name: A-site tRNA_Ile2
Representative chains: X
Length: 76 nucleotides
Theoretical weight: 24.87 KDa
Rfam: tRNA

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