› 8i3p

X-ray diffraction
1.3Å resolution

crystal structure of yeast cytosine deaminase mutant yCD-RQ-1/8SAH in complex with (R)-4-hydroxy-3,4-dihydropyrimidin-2(1H)-one

Released:
DOI: 10.2210/pdb8i3p/pdb
PDB entry 8i3p coloured by chain, front view.
PDB entry 8i3p coloured by chain, side view.
PDB entry 8i3p coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Entry authors: Qin MM, Deng HZ, Yao LS

Function and Biology Details

Reaction catalysed: 
Cytosine + H(2)O = uracil + NH(3)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171310 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosine deaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 22.76 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12178 (Residues: 1-158; Coverage: 100%)
Gene names: FCY1, YP9499.17, YPR062W
Sequence domains: Cytidine and deoxycytidylate deaminase zinc-binding region

Ligands and Environments

3 bound ligands:
Ligand OS0 2 x OS0
Ligand ZN 2 x ZN
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL10U2
Spacegroup: P21
Unit cell:
a: 49.175Å b: 68.9Å c: 49.424Å
α: 90° β: 101.58° γ: 90°
R-values:
R R work R free
0.142 0.141 0.161
Expression system: Escherichia coli BL21(DE3)

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