› 8i4r

X-ray diffraction
2.51Å resolution

Crystal structure of Acyl-CoA dehydrogenase complexed with Acetyl-CoA from Thermobifida fusca

Released:
DOI: 10.2210/pdb8i4r/pdb
PDB entry 8i4r coloured by chain, front view.
PDB entry 8i4r coloured by chain, side view.
PDB entry 8i4r coloured by chain, top view.
Source organism: Thermobifida fusca
Entry authors: Choi M, Seok J, Kim K-J

Function and Biology Details

Reaction catalysed: 
ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-221046 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Succinate--CoA ligase [ADP-forming] subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 43.32 KDa
Source organism: Thermobifida fusca
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A4D5XMK4 (Residues: 293-677; Coverage: 57%)
Gene names: Tfu_2576,2577, sucD
Sequence domains:

Ligands and Environments


Cofactor: Ligand ACO 1 x ACO
1 bound ligand:
Ligand FAD 1 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: I4122
Unit cell:
a: 102.143Å b: 102.143Å c: 312.183Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.195
Expression system: Escherichia coli BL21(DE3)

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