› 8i5c

X-ray diffraction
3.34Å resolution

Crystal structure of a TCR in complex with HLA-A*11:01 bound to KRAS peptide (VVGAVGVGK)

Released:
DOI: 10.2210/pdb8i5c/pdb
PDB entry 8i5c coloured by chain, front view.
PDB entry 8i5c coloured by chain, side view.
PDB entry 8i5c coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a TCR in complex with HLA-A*11:01 bound to KRAS peptide (VVGAVGVGK)
Lu D, Chen Y, Jiang M, Tan SG, Chai Y, Gao GF
Nat Commun (2023)

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-279414 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ig-like domain-containing protein Chains: A, F, K, P, U, Z, e, j, o, t
Molecule details ›
Chains: A, F, K, P, U, Z, e, j, o, t
Length: 274 amino acids
Theoretical weight: 31.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: U5YJJ6 (Residues: 25-298; Coverage: 88%)
Gene name: HLA-A
Sequence domains:
Beta-2-microglobulin Chains: B, G, L, Q, V, a, f, k, p, u
Molecule details ›
Chains: B, G, L, Q, V, a, f, k, p, u
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
GTPase KRas, N-terminally processed Chains: C, H, M, R, W, b, g, l, q, v
Molecule details ›
Chains: C, H, M, R, W, b, g, l, q, v
Length: 9 amino acids
Theoretical weight: 786 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01116 (Residues: 8-16; Coverage: 5%)
Gene names: KRAS, KRAS2, RASK2
TCR alpha chain Chains: D, I, N, S, X, c, h, m, r, w
Molecule details ›
Chains: D, I, N, S, X, c, h, m, r, w
Length: 199 amino acids
Theoretical weight: 22.41 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
TCR beta chain Chains: E, J, O, T, Y, d, i, n, s, x
Molecule details ›
Chains: E, J, O, T, Y, d, i, n, s, x
Length: 242 amino acids
Theoretical weight: 27.03 KDa
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 156.864Å b: 156.889Å c: 191.966Å
α: 77.37° β: 78.13° γ: 82.27°
R-values:
R R work R free
0.226 0.224 0.258
Expression systems:
  • Escherichia coli
  • Not provided

Quick links