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Complex form of MsmUdgX H109K mutant and uracil- obtained from uracil DNA (ttUtt) post its cleavage by MsmUdgX H109K

X-ray diffraction
1.61Å resolution
PDB entry 8iin coloured by chain, front view.
PDB entry 8iin coloured by chain, side view.
PDB entry 8iin coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Type-4 uracil-DNA glycosylase
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Multimeric state: monomeric
Accessible surface area: 9615.74 Å2
Buried surface area: 672.23 Å2
Dissociation area: 147 Å2
Dissociation energy (ΔGdiss): -1.74 kcal/mol
Dissociation entropy (TΔSdiss): 1.38 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-499191

Macromolecules

Type-4 uracil-DNA glycosylase
Chain: A
Length: 206 amino acids
Theoretical weight: 21.81 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0QP43 (Residues: 2-207; Coverage: 99%)
Gene name: MSMEG_0265
Pfam: Uracil DNA glycosylase superfamily
Type-4 uracil-DNA glycosylase in PDB entry 8iin, assembly 1, front view.
Type-4 uracil-DNA glycosylase in PDB entry 8iin, assembly 1, side view.
Type-4 uracil-DNA glycosylase in PDB entry 8iin, assembly 1, top view.
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