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The crystal structure of dGTPalphaSe-Rp:DNApre-II:Pol X substrate ternary complex

X-ray diffraction
3Å resolution
PDB entry 8ile coloured by chain, front view.
PDB entry 8ile coloured by chain, side view.
PDB entry 8ile coloured by chain, top view.
Source organisms:
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct DNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Repair DNA polymerase X and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 18979.9 Å2
Buried surface area: 4344.72 Å2
Dissociation area: 2,043.76 Å2
Dissociation energy (ΔGdiss): -1.76 kcal/mol
Dissociation entropy (TΔSdiss): 31 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-270525

Macromolecules

Repair DNA polymerase X
Chains: A, B
Length: 176 amino acids
Theoretical weight: 20.47 KDa
Source organism: African swine fever virus BA71V
Expression system: Escherichia coli
UniProt:
  • Canonical: P42494 (Residues: 1-174; Coverage: 100%)
Gene names: Ba71V-97, O174L
Pfam:
Repair DNA polymerase X in PDB entry 8ile, assembly 1, front view.
Repair DNA polymerase X in PDB entry 8ile, assembly 1, side view.
Repair DNA polymerase X in PDB entry 8ile, assembly 1, top view.
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DNA chains E, G
Name: DNA (5'-D(*CP*TP*GP*GP*AP*TP*CP*CP*A)-3')
Representative chains: E, G
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 9 nucleotides
Theoretical weight: 2.72 KDa
DNA (5'-D(*CP*TP*GP*GP*AP*TP*CP*CP*A)-3') in PDB entry 8ile, assembly 1, front view.
DNA (5'-D(*CP*TP*GP*GP*AP*TP*CP*CP*A)-3') in PDB entry 8ile, assembly 1, side view.
DNA (5'-D(*CP*TP*GP*GP*AP*TP*CP*CP*A)-3') in PDB entry 8ile, assembly 1, top view.
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