Structure analysis

Wheat 80S ribosome stalled on AUG-Stop boron dependently with cycloheximide

Electron Microscopy
3.4Å resolution
Source organisms:
Assembly composition:
hetero 80-mer (preferred)
Entry contents: 74 distinct polypeptide molecules
6 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 80-mer
PDBe Complex ID: PDB-CPX-278899

Macromolecules

Chain: ba
Length: 137 amino acids
Theoretical weight: 15.74 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6PK86 (Residues: 1-137; Coverage: 100%)
Gene names: CFC21_088220, CFC21_088221
Pfam: Ribosomal protein S24e

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Chain: ca
Length: 225 amino acids
Theoretical weight: 25.34 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6AYT7 (Residues: 1-225; Coverage: 100%)
Gene name: CFC21_017118
Pfam: Ribosomal protein S8e

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Chain: da
Length: 188 amino acids
Theoretical weight: 20.46 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5AUH7 (Residues: 1-188; Coverage: 100%)
Gene name: CFC21_017397
Pfam: Plectin/S10 domain

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Chain: ga
Length: 142 amino acids
Theoretical weight: 15.7 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5FV77 (Residues: 1-142; Coverage: 100%)
Gene names: CFC21_036826, CFC21_036828, CFC21_037201, CFC21_042798, CFC21_048659, CFC21_068535, CFC21_080452
Pfam: Ribosomal protein S12/S23

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Chain: ha
Length: 332 amino acids
Theoretical weight: 36.2 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5D4F5 (Residues: 1-332; Coverage: 100%)
Gene names: CFC21_040720, CFC21_047775, TRAES_3BF007900030CFD_c1
Pfam: WD domain, G-beta repeat

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Chain: ia
Length: 227 amino acids
Theoretical weight: 25.41 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q8L804 (Residues: 1-227; Coverage: 100%)
Pfam:

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Chain: ja
Length: 265 amino acids
Theoretical weight: 30.01 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A1D5SLI4 (Residues: 1-265; Coverage: 100%)
Gene names: CFC21_005863, CFC21_010690
Pfam:

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Chain: ka
Length: 200 amino acids
Theoretical weight: 22.35 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5D067 (Residues: 1-200; Coverage: 100%)
Gene names: CFC21_039914, TRAES_3BF058200010CFD_c1
Pfam: Ribosomal protein S7p/S5e

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Chain: la
Length: 149 amino acids
Theoretical weight: 16.87 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6HX49 (Residues: 1-149; Coverage: 100%)
Gene name: CFC21_052703
Pfam: Ribosomal protein S9/S16

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Chain: ma
Length: 127 amino acids
Theoretical weight: 14.13 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: U5HTD8 (Residues: 1-127; Coverage: 100%)
Gene names: CFC21_019225, CFC21_019228, CFC21_019231, CFC21_019235, CFC21_054980, CFC21_107037, rps20
Pfam: Ribosomal protein S10p/S20e

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Chain: na
Length: 151 amino acids
Theoretical weight: 16.4 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6SS17 (Residues: 1-151; Coverage: 100%)
Gene name: CFC21_104703
Pfam: Ribosomal protein S11

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Chain: oa
Length: 152 amino acids
Theoretical weight: 17.7 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q8L806 (Residues: 1-152; Coverage: 100%)
Gene names: CFC21_068138, CFC21_073936, CFC21_099813
Pfam: Ribosomal protein S13/S18

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Chain: pa
Length: 151 amino acids
Theoretical weight: 17.1 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5XUU9 (Residues: 1-151; Coverage: 100%)
Pfam:

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Chain: qa
Length: 143 amino acids
Theoretical weight: 16.52 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B5YWZ3 (Residues: 1-143; Coverage: 100%)
Gene name: CFC21_006450
Pfam: Ribosomal S17

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Chain: ra
Length: 155 amino acids
Theoretical weight: 17.1 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5FWF5 (Residues: 1-155; Coverage: 100%)
Gene names: CFC21_065464, CFC21_076659
Pfam: Ribosomal protein S19e

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Chain: sa
Length: 154 amino acids
Theoretical weight: 17.42 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5ASA4 (Residues: 1-154; Coverage: 100%)
Gene names: CAMPLR22A2D_LOCUS2419, CFC21_016803, CFC21_029550
Pfam: Ribosomal protein S19

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Chain: ta
Length: 108 amino acids
Theoretical weight: 12.09 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5EP45 (Residues: 1-108; Coverage: 100%)
Gene names: CFC21_009979, CFC21_056005, CFC21_056010, CFC21_056015
Pfam: S25 ribosomal protein

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Chain: ua
Length: 86 amino acids
Theoretical weight: 9.82 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q7X9K4 (Residues: 1-86; Coverage: 100%)
Pfam: Ribosomal protein S28e

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Chain: va
Length: 129 amino acids
Theoretical weight: 14.92 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A077RZD1 (Residues: 1-129; Coverage: 100%)
Gene names: CFC21_034863, CFC21_040826, CFC21_046784, TRAES_3BF007400090CFD_c1
Pfam: Ribosomal protein S8

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Chain: wa
Length: 56 amino acids
Theoretical weight: 6.39 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7K3 (Residues: 1-56; Coverage: 100%)
Gene name: RPS29
Pfam: Ribosomal protein S14p/S29e

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Chain: xa
Length: 86 amino acids
Theoretical weight: 9.58 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A341UET3 (Residues: 5-90; Coverage: 96%)
Gene names: CFC21_053114, CFC21_060788
Pfam: Ribosomal protein S27

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Chain: ya
Length: 62 amino acids
Theoretical weight: 6.93 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6S9M7 (Residues: 1-62; Coverage: 100%)
Gene name: CFC21_101292
Pfam: Ribosomal protein S30

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Chain: za
Length: 308 amino acids
Theoretical weight: 33.3 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5E2W7 (Residues: 1-308; Coverage: 100%)
Gene name: CFC21_058947
Pfam: Ribosomal protein S2

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Chain: bb
Length: 263 amino acids
Theoretical weight: 30.04 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6IU74 (Residues: 1-263; Coverage: 100%)
Gene name: CFC21_058573
Pfam: Ribosomal S3Ae family

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Chain: cb
Length: 82 amino acids
Theoretical weight: 9.19 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5EL62 (Residues: 1-82; Coverage: 100%)
Gene names: CFC21_052197, CFC21_061690
Pfam: Ribosomal protein S21e

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Chain: db
Length: 156 amino acids
Theoretical weight: 16.58 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6LWE1 (Residues: 1-156; Coverage: 100%)
Gene name: CFC21_074379
Pfam: Ribosomal protein S26e

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Chain: eb
Length: 195 amino acids
Theoretical weight: 22.53 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5FPA7 (Residues: 1-195; Coverage: 100%)
Gene names: CFC21_011030, CFC21_064815, CFC21_070616
Pfam:

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Chain: fb
Length: 274 amino acids
Theoretical weight: 30 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6LVN2 (Residues: 1-274; Coverage: 100%)
Gene name: CFC21_074258
Pfam:

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Chain: gb
Length: 250 amino acids
Theoretical weight: 28.49 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A2X0SLI3 (Residues: 1-250; Coverage: 100%)
Gene names: CAMPLR22A2D_LOCUS1521, CFC21_015985, CFC21_028704
Pfam: Ribosomal protein S6e

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Chain: hb
Length: 192 amino acids
Theoretical weight: 22.3 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7K2 (Residues: 1-192; Coverage: 100%)
Gene names: CFC21_051939, CFC21_057885
Pfam: Ribosomal protein S7e

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Chain: ib
Length: 159 amino acids
Theoretical weight: 17.84 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5BFB7 (Residues: 1-159; Coverage: 100%)
Gene names: CFC21_025034, CFC21_031339
Pfam:

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Chain: AA
Length: 258 amino acids
Theoretical weight: 29.41 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6RED0 (Residues: 1-258; Coverage: 100%)
Gene name: CFC21_097640
Pfam: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

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Chain: BA
Length: 164 amino acids
Theoretical weight: 18.72 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: F4YWL9 (Residues: 1-164; Coverage: 100%)
Gene names: CFC21_068953, RPL21
Pfam: Ribosomal protein L21e

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Chain: CA
Length: 137 amino acids
Theoretical weight: 15.62 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A341XZK6 (Residues: 1-137; Coverage: 100%)
Gene name: CFC21_098384
Pfam:

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Chain: DA
Length: 261 amino acids
Theoretical weight: 28.25 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5ECL2 (Residues: 1-261; Coverage: 100%)
Gene names: CFC21_052694, CFC21_057232, CFC21_061205, CFC21_064588, CFC21_070369, CFC21_076331
Pfam:

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Chain: EA
Length: 180 amino acids
Theoretical weight: 20.73 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B5XX39 (Residues: 1-180; Coverage: 100%)
Pfam:

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Chain: FA
Length: 189 amino acids
Theoretical weight: 21.33 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5I5G5 (Residues: 1-189; Coverage: 100%)
Gene names: CFC21_033307, CFC21_038784, CFC21_095138, TRAES_3BF060500370CFD_c1
Pfam: Ribosomal protein L6

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Chain: GA
Length: 140 amino acids
Theoretical weight: 15.04 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7K4 (Residues: 1-140; Coverage: 100%)
Gene names: CFC21_006360, CFC21_011199, CFC21_085267, CFC21_090119
Pfam: Ribosomal protein L14p/L23e

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Chain: HA
Length: 204 amino acids
Theoretical weight: 24.49 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5FEX7 (Residues: 1-204; Coverage: 100%)
Gene names: CAMPLR22A2D_LOCUS2234, CFC21_029414, CFC21_065333, CFC21_077061
Pfam: Ribosomal L15

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Chain: IA
Length: 145 amino acids
Theoretical weight: 16.05 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6DCS5 (Residues: 1-145; Coverage: 100%)
Gene name: CFC21_028780
Pfam: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A

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Chain: JA
Length: 187 amino acids
Theoretical weight: 21.29 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6ATQ1 (Residues: 1-187; Coverage: 100%)
Gene name: CFC21_015448
Pfam: Ribosomal protein 60S L18 and 50S L18e

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Chain: KA
Length: 301 amino acids
Theoretical weight: 34.49 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5BZZ2 (Residues: 1-301; Coverage: 100%)
Gene names: CFC21_017319, CFC21_030053
Pfam:

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Chain: LA
Length: 213 amino acids
Theoretical weight: 24.69 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6DA12 (Residues: 1-213; Coverage: 100%)
Gene names: CFC21_015301, CFC21_027941
Pfam: Ribosomal protein L19e

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Chain: MA
Length: 170 amino acids
Theoretical weight: 19.53 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5FSM8 (Residues: 1-170; Coverage: 100%)
Gene names: CFC21_071208, CFC21_077189
Pfam: Ribosomal protein L22p/L17e

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Chain: NA
Length: 152 amino acids
Theoretical weight: 16.92 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A1D5UQZ5 (Residues: 1-152; Coverage: 100%)
Gene names: CAMPLR22A2D_LOCUS3567, CFC21_010645, CFC21_024362, CFC21_030666
Pfam:

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Chain: OA
Length: 162 amino acids
Theoretical weight: 18.45 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5CDW4 (Residues: 1-162; Coverage: 100%)
Gene name: CFC21_042648
Pfam: Ribosomal protein L24e

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Chain: PA
Length: 157 amino acids
Theoretical weight: 17.59 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6GQI2 (Residues: 1-157; Coverage: 100%)
Gene name: CFC21_046528
Pfam:

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Chain: QA
Length: 147 amino acids
Theoretical weight: 16.61 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B5ZZN9 (Residues: 1-147; Coverage: 100%)
Pfam: Ribosomal L28e protein family

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Chain: RA
Length: 112 amino acids
Theoretical weight: 12.37 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7K9 (Residues: 1-112; Coverage: 100%)
Pfam: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

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Chain: SA
Length: 123 amino acids
Theoretical weight: 14.15 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5G990 (Residues: 1-123; Coverage: 100%)
Gene names: CFC21_084218, CFC21_093418
Pfam: Ribosomal protein L31e

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Chain: TA
Length: 133 amino acids
Theoretical weight: 15.74 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6SDD9 (Residues: 1-133; Coverage: 100%)
Gene name: CFC21_102549
Pfam: Ribosomal protein L32

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Chain: UA
Length: 93 amino acids
Theoretical weight: 10.77 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A1D6BC85 (Residues: 1-93; Coverage: 100%)
Gene names: CFC21_086135, CFC21_091005, CFC21_091010
Pfam: Ribosomal protein L37e

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Chain: VA
Length: 76 amino acids
Theoretical weight: 9.44 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: M7ZU91 (Residues: 1-76; Coverage: 100%)
Gene name: TRIUR3_17747
Pfam: Ribosomal L39 protein

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Chain: WA
Length: 105 amino acids
Theoretical weight: 12.14 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5HX73 (Residues: 1-105; Coverage: 100%)
Gene names: CAMPLR22A2D_LOCUS2111, CFC21_023035, CFC21_066964, CFC21_067977, CFC21_072777, CFC21_073788, CFC21_078767, CFC21_079793, CFC21_099667, CFC21_099668, CFC21_110912
Pfam: Ribosomal protein L44

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Chain: XA
Length: 135 amino acids
Theoretical weight: 15.49 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6DGT8 (Residues: 1-135; Coverage: 100%)
Gene name: CFC21_030928
Pfam: Ribosomal protein L14

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Chain: YA
Length: 178 amino acids
Theoretical weight: 21.38 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5D7B8 (Residues: 1-178; Coverage: 100%)
Gene name: CFC21_041679
Pfam: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A

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Chain: ZA
Length: 130 amino acids
Theoretical weight: 14.5 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6C0D4 (Residues: 1-130; Coverage: 100%)
Gene name: CFC21_021810
Pfam: Ribosomal L22e protein family

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Chain: AB
Length: 112 amino acids
Theoretical weight: 12.56 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7L5 (Residues: 1-112; Coverage: 100%)
Gene name: CFC21_064117
Pfam: Ribosomal protein L36e

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Chain: BB
Length: 124 amino acids
Theoretical weight: 14.4 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q8L805 (Residues: 1-124; Coverage: 100%)
Gene name: RPL35
Pfam: Ribosomal L29 protein

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Chain: CB
Length: 244 amino acids
Theoretical weight: 28.16 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6DM38 (Residues: 1-244; Coverage: 100%)
Gene name: CFC21_075603
Pfam:

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Chain: DB
Length: 389 amino acids
Theoretical weight: 44.72 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q7X748 (Residues: 1-389; Coverage: 100%)
Gene names: CFC21_070698, RPL3, RPL3-A2, RPL3A-2
Pfam: Ribosomal protein L3

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Chain: EB
Length: 404 amino acids
Theoretical weight: 44.29 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B5ZXX7 (Residues: 1-404; Coverage: 100%)
Gene name: CFC21_012388
Pfam:

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Chain: FB
Length: 206 amino acids
Theoretical weight: 23.58 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7L1 (Residues: 1-206; Coverage: 100%)
Pfam: Ribosomal protein L13

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Chain: GB
Length: 92 amino acids
Theoretical weight: 10.27 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: D8L9P6 (Residues: 1-92; Coverage: 100%)
Gene names: CFC21_098386, CFC21_109870, TAA_ctg0616b.00240.1, TRAES_3BF073800030CFD_c1
Pfam: Ribosomal L37ae protein family

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Chain: HB
Length: 217 amino acids
Theoretical weight: 24.52 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6HUM3 (Residues: 1-217; Coverage: 100%)
Gene name: CFC21_052842
Pfam: Ribosomal protein L16p/L10e

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Chain: IB
Length: 25 amino acids
Theoretical weight: 3.45 KDa
Source organism: Oryza sativa
UniProt:
  • Canonical: P62125 (Residues: 1-25; Coverage: 100%)
Gene names: LOC_Os03g56878, OSJNBa0091J19.8, Os03g0781500, Os03g0781501, RPL41
Pfam: Ribosomal protein L41

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Chain: JB
Length: 129 amino acids
Theoretical weight: 14.83 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A1D5XNC2 (Residues: 1-129; Coverage: 100%)
Gene names: CFC21_051579, CFC21_053842, CFC21_056000, CFC21_060038, CFC21_062406
Pfam:

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Chain: KB
Length: 208 amino acids
Theoretical weight: 24.19 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6NJZ9 (Residues: 1-208; Coverage: 100%)
Gene name: CFC21_081845
Pfam: Ribosomal protein L13e

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Chain: LB
Length: 219 amino acids
Theoretical weight: 24.58 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6QGW3 (Residues: 14-232; Coverage: 94%)
Gene name: CFC21_092553
Pfam: Ribosomal protein L6e

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Chain: MB
Length: 112 amino acids
Theoretical weight: 12.7 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A0C4BK99 (Residues: 1-112; Coverage: 100%)
Gene name: CFC21_089636
Pfam: Ribosomal protein L35Ae

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Chain: NB
Length: 69 amino acids
Theoretical weight: 8.07 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A1D5W6Q2 (Residues: 1-69; Coverage: 100%)
Gene names: CFC21_043473, CFC21_044750
Pfam: Ribosomal L38e protein family

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Chain: OB
Length: 60 amino acids
Theoretical weight: 6.99 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A1D5RRD3 (Residues: 1-60; Coverage: 100%)
Gene names: CFC21_007097, CFC21_011996
Pfam: Ribosomal L29e protein family

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Chain: PB
Length: 119 amino acids
Theoretical weight: 13.8 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6RFY8 (Residues: 1-119; Coverage: 100%)
Gene name: CFC21_098055
Pfam: Ribosomal protein L34e

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Chain: bl
Length: 437 amino acids
Theoretical weight: 49.05 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: I3NM28 (Residues: 1-437; Coverage: 100%)
Gene names: 4N5.2, CFC21_060346
Pfam:

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Name: 18S Ribosomal RNA
Representative chains: aa
Length: 1810 nucleotides
Theoretical weight: 583.61 KDa
Rfam: Eukaryotic small subunit ribosomal RNA

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Name: 60S ribosomal RNA
Representative chains: RB
Length: 3386 nucleotides
Theoretical weight: 1095.66 KDa
Rfam: Eukaryotic large subunit ribosomal RNA

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Name: 5.8S ribosomal RNA
Representative chains: SB
Length: 160 nucleotides
Theoretical weight: 51.54 KDa
Rfam: 5.8S ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: TB
Length: 120 nucleotides
Theoretical weight: 38.72 KDa
Rfam: 5S ribosomal RNA

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Name: Messenger RNA
Representative chains: al
Length: 7 nucleotides
Theoretical weight: 2.23 KDa

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Name: tRNAi
Representative chains: cl
Length: 75 nucleotides
Theoretical weight: 24.45 KDa
Rfam: tRNA

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