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Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the resting state

Electron Microscopy
3.28Å resolution
PDB entry 8j5r coloured by chain, front view.
PDB entry 8j5r coloured by chain, side view.
PDB entry 8j5r coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Assembly composition:
hetero tetramer (preferred)
Entry contents: 4 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero tetramer
Accessible surface area: 73793.34 Å2
Buried surface area: 10719.07 Å2
Dissociation area: 1,305.43 Å2
Dissociation energy (ΔGdiss): 3.46 kcal/mol
Dissociation entropy (TΔSdiss): 15.13 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-242610

Macromolecules

Uncharacterized protein Rv1280c
Chain: A
Length: 599 amino acids
Theoretical weight: 64.39 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: P9WGU5 (Residues: 1-591; Coverage: 100%)
Gene names: MTCY50.02, Rv1280c
Pfam: Bacterial extracellular solute-binding proteins, family 5 Middle
Uncharacterized protein Rv1280c in PDB entry 8j5r, assembly 1, front view.
Uncharacterized protein Rv1280c in PDB entry 8j5r, assembly 1, side view.
Uncharacterized protein Rv1280c in PDB entry 8j5r, assembly 1, top view.
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Putative peptide transport permease protein Rv1283c
Chain: B
Length: 325 amino acids
Theoretical weight: 35.12 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: P9WFZ7 (Residues: 1-325; Coverage: 100%)
Gene names: MTCY373.02c, Rv1283c
Pfam:
Putative peptide transport permease protein Rv1283c in PDB entry 8j5r, assembly 1, front view.
Putative peptide transport permease protein Rv1283c in PDB entry 8j5r, assembly 1, side view.
Putative peptide transport permease protein Rv1283c in PDB entry 8j5r, assembly 1, top view.
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Putative peptide transport permease protein Rv1282c
Chain: C
Length: 291 amino acids
Theoretical weight: 31.4 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: P9WFZ9 (Residues: 1-291; Coverage: 100%)
Gene names: MTCY373.01c, MTCY3H3.01, Rv1282c
Pfam:
Putative peptide transport permease protein Rv1282c in PDB entry 8j5r, assembly 1, front view.
Putative peptide transport permease protein Rv1282c in PDB entry 8j5r, assembly 1, side view.
Putative peptide transport permease protein Rv1282c in PDB entry 8j5r, assembly 1, top view.
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Uncharacterized ABC transporter ATP-binding protein Rv1281c
Chain: D
Length: 612 amino acids
Theoretical weight: 65.37 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: P9WQJ5 (Residues: 1-612; Coverage: 100%)
Gene names: MTCY50.01, Rv1281c
Pfam:
Uncharacterized ABC transporter ATP-binding protein Rv1281c in PDB entry 8j5r, assembly 1, front view.
Uncharacterized ABC transporter ATP-binding protein Rv1281c in PDB entry 8j5r, assembly 1, side view.
Uncharacterized ABC transporter ATP-binding protein Rv1281c in PDB entry 8j5r, assembly 1, top view.
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