› 8j6k

X-ray diffraction
3.12Å resolution

Crystal structure of pro-interleukin-18 and caspase-4 complex

Released:
DOI: 10.2210/pdb8j6k/pdb
PDB entry 8j6k coloured by chain, front view.
PDB entry 8j6k coloured by chain, side view.
PDB entry 8j6k coloured by chain, top view.
Source organisms:
Primary publication:
Recognition and maturation of IL-18 by caspase-4 noncanonical inflammasome.
Shi X, Sun Q, Hou Y, Zeng H, Cao Y, Dong M, Ding J, Shao F
Nature (2023)
PMID: 37993714

Function and Biology Details

Reaction catalysed: 
Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-235982 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Caspase-4 subunit p20 Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49662 (Residues: 102-270; Coverage: 45%)
Gene names: CASP4, ICH2
Sequence domains: Caspase domain
Caspase-4 subunit p10 Chain: a
Molecule details ›
Chain: a
Length: 88 amino acids
Theoretical weight: 10.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49662 (Residues: 290-377; Coverage: 23%)
Gene names: CASP4, ICH2
Sequence domains: Caspase domain
Interleukin-18 Chain: B
Molecule details ›
Chain: B
Length: 194 amino acids
Theoretical weight: 22.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14116 (Residues: 1-193; Coverage: 100%)
Gene names: IGIF, IL18, IL1F4
Sequence domains: Interleukin-1 / 18
Arginine ADP-riboxanase OspC3 Chain: C
Molecule details ›
Chain: C
Length: 161 amino acids
Theoretical weight: 18.37 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2US87 (Residues: 332-484; Coverage: 32%)
Gene names: SF_p0115, ospC3
Sequence domains: Shigella flexneri OspC protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL02U1
Spacegroup: P3121
Unit cell:
a: 86.254Å b: 86.254Å c: 194.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.238 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links