› 8j6l

Electron Microscopy
3.05Å resolution

Cryo-EM structure of thehydroxycarboxylic acid receptor 2-Gi protein complex bound niacin

Released:
DOI: 10.2210/pdb8j6l/pdb
PDB entry 8j6l coloured by chain, front view.
PDB entry 8j6l coloured by chain, side view.
PDB entry 8j6l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular recognition of niacin and lipid-lowering drugs by the human hydroxycarboxylic acid receptor 2.
Zhu S, Yuan Q, Li X, He X, Shen S, Wang D, Li J, Cheng X, Duan X, Xu HE, Duan J
Cell Rep 42 113406 (2023)
PMID: 37952153
Related structures: EMD-36007

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-273985 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.41 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 370 amino acids
Theoretical weight: 40.1 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 70 amino acids
Theoretical weight: 7.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 2-71; Coverage: 99%)
Gene name: GNG2
Sequence domains: GGL domain
SVF16 Chain: H
Molecule details ›
Chain: H
Length: 247 amino acids
Theoretical weight: 26.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Hydroxycarboxylic acid receptor 2 Chain: R
Molecule details ›
Chain: R
Length: 488 amino acids
Theoretical weight: 56.11 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TDS4 (Residues: 1-331; Coverage: 91%)
Gene names: GPR109A, HCA2, HCAR2, HM74A, NIACR1
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:
Ligand NIO 1 x NIO
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.05Å
Relevant EMDB volumes: EMD-36007
Expression system: Spodoptera frugiperda

Quick links

Citations

1 citations in other articles

Structure-guided engineering of biased-agonism in the human niacin receptor via single amino acid substitution.
Yadav et al. (2024)