› 8jbg

Electron Microscopy
2.8Å resolution

Neurokinin B bound to active human neurokinin 3 receptor in complex with Gq

Released:
DOI: 10.2210/pdb8jbg/pdb
PDB entry 8jbg coloured by chain, front view.
PDB entry 8jbg coloured by chain, side view.
PDB entry 8jbg coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into neurokinin 3 receptor activation by endogenous and analogue peptide agonists.
Sun W, Yang F, Zhang H, Yuan Q, Ling S, Wang Y, Lv P, Li Z, Luo Y, Liu D, Yin W, Shi P, Xu HE, Tian C
OpenAccess logo Cell Discov 9 66 (2023)
PMID: 37391393
Related structures: EMD-36145

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-276190 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Neuromedin-K receptor Chain: B
Molecule details ›
Chain: B
Length: 1428 amino acids
Theoretical weight: 158.09 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P29371 (Residues: 1-394; Coverage: 85%)
Gene names: NK3R, TAC3R, TACR3
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: D
Molecule details ›
Chain: D
Length: 377 amino acids
Theoretical weight: 41.06 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
ScFv16 nanobody Chain: E
Molecule details ›
Chain: E
Length: 304 amino acids
Theoretical weight: 32.71 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Neurokinin-B Chain: A
Molecule details ›
Chain: A
Length: 10 amino acids
Theoretical weight: 1.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHF0 (Residues: 81-90; Coverage: 10%)
Gene names: NKNB, TAC3, UNQ585/PRO1155
Guanine nucleotide-binding protein Gq (G324) Chain: C
Molecule details ›
Chain: C
Length: 361 amino acids
Theoretical weight: 41.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-36145
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 review citation

Neuroimmune communication in allergic rhinitis.
Zhou et al. (2023)