› 8jiw

Electron Microscopy
2.88Å resolution

Atomic structure of wheat ribosome reveals unique features of the plant ribosomes

Released:
DOI: 10.2210/pdb8jiw/pdb
PDB entry 8jiw coloured by chain, front view.
PDB entry 8jiw coloured by chain, side view.
PDB entry 8jiw coloured by chain, top view.
Source organism: Triticum aestivum
Primary publication:
Cryo-EM structure of wheat ribosome reveals unique features of the plant ribosomes.
Mishra RK, Sharma P, Khaja FT, Uday AB, Hussain T
Structure (2024)
PMID: 38458197
Related structures: EMD-36332

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero 33-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-495577 (preferred)
Entry contents:
32 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (33 distinct):
Small ribosomal subunit protein uS2 Chain: BA
Molecule details ›
Chain: BA
Length: 304 amino acids
Theoretical weight: 32.93 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6AV88 (Residues: 1-304; Coverage: 100%)
Gene name: CFC21_016030
Sequence domains:
Small ribosomal subunit protein eS1 Chain: BB
Molecule details ›
Chain: BB
Length: 263 amino acids
Theoretical weight: 30.04 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6IU74 (Residues: 1-263; Coverage: 100%)
Gene name: CFC21_058573
Sequence domains: Ribosomal S3Ae family
S5 DRBM domain-containing protein Chain: BC
Molecule details ›
Chain: BC
Length: 279 amino acids
Theoretical weight: 30.48 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6N0N5 (Residues: 1-279; Coverage: 100%)
Gene name: CFC21_080285
Sequence domains:
40S ribosomal protein S4 Chain: BE
Molecule details ›
Chain: BE
Length: 265 amino acids
Theoretical weight: 30.01 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A1D5SLI4 (Residues: 1-265; Coverage: 100%)
Gene names: CFC21_005863, CFC21_010690
Sequence domains:
40S ribosomal protein S6 Chain: BG
Molecule details ›
Chain: BG
Length: 250 amino acids
Theoretical weight: 28.52 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5C8N6 (Residues: 1-250; Coverage: 100%)
Gene names: CAMPLR22A2D_LOCUS542, CFC21_015030, CFC21_021276, CFC21_021277
Sequence domains: Ribosomal protein S6e
40S ribosomal protein S7 Chain: BH
Molecule details ›
Chain: BH
Length: 192 amino acids
Theoretical weight: 22.3 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q5I7K2 (Residues: 1-192; Coverage: 100%)
Gene names: CFC21_051939, CFC21_057885
Sequence domains: Ribosomal protein S7e
40S ribosomal protein S8 Chain: BI
Molecule details ›
Chain: BI
Length: 224 amino acids
Theoretical weight: 25.19 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6C6G0 (Residues: 1-224; Coverage: 100%)
Gene name: CFC21_023528
Sequence domains: Ribosomal protein S8e
30S ribosomal protein S4, chloroplastic Chain: BJ
Molecule details ›
Chain: BJ
Length: 195 amino acids
Theoretical weight: 22.53 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5FPA7 (Residues: 1-195; Coverage: 100%)
Gene names: CFC21_011030, CFC21_064815, CFC21_070616
Sequence domains:
Small ribosomal subunit protein uS17 N-terminal domain-containing protein Chain: BL
Molecule details ›
Chain: BL
Length: 161 amino acids
Theoretical weight: 18.14 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6QHW3 (Residues: 1-161; Coverage: 100%)
Gene name: CFC21_092816
Sequence domains:
Small ribosomal subunit protein uS15 N-terminal domain-containing protein Chain: BN
Molecule details ›
Chain: BN
Length: 151 amino acids
Theoretical weight: 17.13 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5I035 (Residues: 1-151; Coverage: 100%)
Gene names: CFC21_054424, CFC21_098222, CFC21_103236, CFC21_103237, CFC21_103240, CFC21_109586
Sequence domains:
30S ribosomal protein S11, chloroplastic Chain: BO
Molecule details ›
Chain: BO
Length: 151 amino acids
Theoretical weight: 16.4 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6SS17 (Residues: 1-151; Coverage: 100%)
Gene name: CFC21_104703
Sequence domains: Ribosomal protein S11
40S ribosomal protein S17 Chain: BR
Molecule details ›
Chain: BR
Length: 143 amino acids
Theoretical weight: 16.48 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B5XY60 (Residues: 1-143; Coverage: 100%)
Sequence domains: Ribosomal S17
40S ribosomal protein S21 Chain: BV
Molecule details ›
Chain: BV
Length: 82 amino acids
Theoretical weight: 9.19 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5EL62 (Residues: 1-82; Coverage: 100%)
Gene names: CFC21_052197, CFC21_061690
Sequence domains: Ribosomal protein S21e
Small ribosomal subunit protein uS8c Chain: BW
Molecule details ›
Chain: BW
Length: 130 amino acids
Theoretical weight: 14.82 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: E2F3W4 (Residues: 1-130; Coverage: 100%)
Gene names: CFC21_007684, CFC21_012582, CFC21_042841, CFC21_048691, TRAES_3BF117500090CFD_c1
Sequence domains: Ribosomal protein S8
40S ribosomal protein S23 Chain: BX
Molecule details ›
Chain: BX
Length: 142 amino acids
Theoretical weight: 15.7 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5FV77 (Residues: 1-142; Coverage: 100%)
Gene names: CFC21_036826, CFC21_036828, CFC21_037201, CFC21_042798, CFC21_048659, CFC21_068535, CFC21_080452
Sequence domains: Ribosomal protein S12/S23
40S ribosomal protein S24 Chain: BY
Molecule details ›
Chain: BY
Length: 137 amino acids
Theoretical weight: 15.74 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6PK86 (Residues: 1-137; Coverage: 100%)
Gene names: CFC21_088220, CFC21_088221
Sequence domains: Ribosomal protein S24e
40S ribosomal protein S26 Chain: Ba
Molecule details ›
Chain: Ba
Length: 139 amino acids
Theoretical weight: 15.32 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6KQB8 (Residues: 1-139; Coverage: 100%)
Gene names: CFC21_068482, CFC21_068483
Sequence domains: Ribosomal protein S26e
40S ribosomal protein S27 Chain: Bb
Molecule details ›
Chain: Bb
Length: 86 amino acids
Theoretical weight: 9.58 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A341UET3 (Residues: 5-90; Coverage: 96%)
Gene names: CFC21_053114, CFC21_060788
Sequence domains: Ribosomal protein S27
40S ribosomal protein S30 Chain: Be
Molecule details ›
Chain: Be
Length: 62 amino acids
Theoretical weight: 6.93 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6S9M7 (Residues: 1-62; Coverage: 100%)
Gene name: CFC21_101292
Sequence domains: Ribosomal protein S30
Small ribosomal subunit protein eS32 Chain: Cn
Molecule details ›
Chain: Cn
Length: 25 amino acids
Theoretical weight: 3.45 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P62124 (Residues: 1-25; Coverage: 100%)
Gene name: RPL41
Sequence domains: Ribosomal protein L41
30S ribosomal protein S3, chloroplastic Chain: BD
Molecule details ›
Chain: BD
Length: 227 amino acids
Theoretical weight: 25.4 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5I1R7 (Residues: 1-227; Coverage: 100%)
Gene names: CFC21_034353, CFC21_034354, CFC21_109361
Sequence domains:
Small ribosomal subunit protein uS7c Chain: BF
Molecule details ›
Chain: BF
Length: 200 amino acids
Theoretical weight: 22.35 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5D067 (Residues: 1-200; Coverage: 100%)
Gene names: CFC21_039914, TRAES_3BF058200010CFD_c1
Sequence domains: Ribosomal protein S7p/S5e
Plectin/eS10 N-terminal domain-containing protein Chain: BK
Molecule details ›
Chain: BK
Length: 188 amino acids
Theoretical weight: 20.46 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5AUH7 (Residues: 1-188; Coverage: 100%)
Gene name: CFC21_017397
Sequence domains: Plectin/S10 domain
40S ribosomal protein S15 Chain: BP
Molecule details ›
Chain: BP
Length: 153 amino acids
Theoretical weight: 17.3 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6LV48 (Residues: 1-153; Coverage: 100%)
Gene name: CFC21_074055
Sequence domains: Ribosomal protein S19
40S ribosomal protein S16 Chain: BQ
Molecule details ›
Chain: BQ
Length: 149 amino acids
Theoretical weight: 16.87 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6HX49 (Residues: 1-149; Coverage: 100%)
Gene name: CFC21_052703
Sequence domains: Ribosomal protein S9/S16
40S ribosomal protein S18 Chain: BS
Molecule details ›
Chain: BS
Length: 152 amino acids
Theoretical weight: 17.68 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6TWW5 (Residues: 1-152; Coverage: 100%)
Gene name: CFC21_111051
Sequence domains: Ribosomal protein S13/S18
40S ribosomal protein S19 Chain: BT
Molecule details ›
Chain: BT
Length: 155 amino acids
Theoretical weight: 17.1 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5FWF5 (Residues: 1-155; Coverage: 100%)
Gene names: CFC21_065464, CFC21_076659
Sequence domains: Ribosomal protein S19e
Small ribosomal subunit protein uS10 domain-containing protein Chain: BU
Molecule details ›
Chain: BU
Length: 117 amino acids
Theoretical weight: 13.04 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6MKF1 (Residues: 11-127; Coverage: 92%)
Gene name: CFC21_069782
Sequence domains: Ribosomal protein S10p/S20e
40S ribosomal protein S28 Chain: Bc
Molecule details ›
Chain: Bc
Length: 65 amino acids
Theoretical weight: 7.48 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: W5E8X2 (Residues: 1-65; Coverage: 100%)
Gene names: CFC21_038625, CFC21_038626, CFC21_050693, CFC21_052484, CFC21_052485, CFC21_061583
Sequence domains: Ribosomal protein S28e
40S ribosomal protein S29 Chain: Bd
Molecule details ›
Chain: Bd
Length: 56 amino acids
Theoretical weight: 6.42 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A1D5ZKM9 (Residues: 1-56; Coverage: 100%)
Gene names: CFC21_067854, CFC21_067862, CFC21_067864, CFC21_070567
Sequence domains: Ribosomal protein S14p/S29e
Mitogen-activated protein kinase Chain: Bg
Molecule details ›
Chain: Bg
Length: 335 amino acids
Theoretical weight: 36.31 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B5Z4Q0 (Residues: 1-335; Coverage: 100%)
Gene name: CFC21_008707
Sequence domains: WD domain, G-beta repeat
40S ribosomal protein S25 Chain: BZ
Molecule details ›
Chain: BZ
Length: 98 amino acids
Theoretical weight: 10.86 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: A0A3B6HY18 (Residues: 1-98; Coverage: 100%)
Gene name: CFC21_053846
Sequence domains: S25 ribosomal protein
18S Ribosomal RNA Chain: Ad
Molecule details ›
Chain: Ad
Length: 1811 nucleotides
Theoretical weight: 584.38 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA

Ligands and Environments

3 bound ligands:
Ligand K 16 x K
Ligand MG 70 x MG
Ligand ZN 1 x ZN
6 modified residues:
Ligand A2M 10 x A2M
Ligand OMC 4 x OMC
Ligand PSU 24 x PSU
Ligand OMU 3 x OMU
Ligand OMG 3 x OMG
Ligand MA6 2 x MA6

Experiments and Validation Details

Entry percentile scores
Resolution: 2.88Å
Relevant EMDB volumes: EMD-36332

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