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Crystal structure of the Green fluorescent protein SE_A277 variant at pH 7.5

X-ray diffraction
2.1Å resolution
PDB entry 8jki coloured by chain, front view.
PDB entry 8jki coloured by chain, side view.
PDB entry 8jki coloured by chain, top view.
Source organism: Aequorea victoria
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Green fluorescent protein
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Multimeric state: monomeric
Accessible surface area: 10239.64 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-154537
Assembly 2
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Assembly Name: Green fluorescent protein
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Multimeric state: monomeric
Accessible surface area: 10178.78 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-154537
Assembly 3
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Assembly Name: Green fluorescent protein
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Multimeric state: monomeric
Accessible surface area: 10372.66 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-154537
Assembly 4
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Assembly Name: Green fluorescent protein
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Multimeric state: monomeric
Accessible surface area: 10391.51 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-154537

Macromolecules

Green fluorescent protein
Chains: A, B, C, D
Length: 236 amino acids
Theoretical weight: 26.96 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Pfam: Green fluorescent protein
Green fluorescent protein in PDB entry 8jki, assembly 1, front view.
Green fluorescent protein in PDB entry 8jki, assembly 1, side view.
Green fluorescent protein in PDB entry 8jki, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P42212  
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