› 8jpt

X-ray diffraction
3.26Å resolution

Crystal Structure of the acyltransferase domain from the eighth module of the spinosad polyketide synthase

Released:
DOI: 10.2210/pdb8jpt/pdb
PDB entry 8jpt coloured by chain, front view.
PDB entry 8jpt coloured by chain, side view.
PDB entry 8jpt coloured by chain, top view.
Source organism: Saccharopolyspora spinosa
Entry authors: Huang S, Zheng J

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246097 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase extender modules 8-10 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 418 amino acids
Theoretical weight: 44.11 KDa
Source organism: Saccharopolyspora spinosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ALM2 (Residues: 473-890; Coverage: 8%)
Gene names: A8926_6425, spnE
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2
Unit cell:
a: 196.345Å b: 154.723Å c: 65.209Å
α: 90° β: 92.09° γ: 90°
R-values:
R R work R free
0.201 0.198 0.258
Expression system: Escherichia coli BL21(DE3)

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