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Crystal Structure of the acyltransferase domain from the eighth module of the spinosad polyketide synthase

X-ray diffraction
3.26Å resolution
PDB entry 8jpt coloured by chain, front view.
PDB entry 8jpt coloured by chain, side view.
PDB entry 8jpt coloured by chain, top view.
Source organism: Saccharopolyspora spinosa
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Polyketide synthase extender modules 8-10
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Multimeric state: monomeric
Accessible surface area: 16308.5 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-275303
Assembly 2
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Assembly Name: Polyketide synthase extender modules 8-10
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Multimeric state: monomeric
Accessible surface area: 16526.46 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-275303
Assembly 3
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Assembly Name: Polyketide synthase extender modules 8-10
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Multimeric state: monomeric
Accessible surface area: 16524.23 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-275303

Macromolecules

Polyketide synthase extender modules 8-10
Chains: A, B, C
Length: 418 amino acids
Theoretical weight: 44.11 KDa
Source organism: Saccharopolyspora spinosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ALM2 (Residues: 473-890; Coverage: 8%)
Gene names: A8926_6425, spnE
Pfam:
Polyketide synthase extender modules 8-10 in PDB entry 8jpt, assembly 1, front view.
Polyketide synthase extender modules 8-10 in PDB entry 8jpt, assembly 1, side view.
Polyketide synthase extender modules 8-10 in PDB entry 8jpt, assembly 1, top view.
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