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Cryo-EM structure of Mycobacterium tuberculosis ATP synthase Fo in complex with TBAJ-587

Electron Microscopy
3.17Å resolution
PDB entry 8jr1 coloured by chain, front view.
PDB entry 8jr1 coloured by chain, side view.
PDB entry 8jr1 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Assembly composition:
hetero decamer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: ATP synthase
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Multimeric state: hetero decamer
Accessible surface area: 32299.32 Å2
Buried surface area: 34424.04 Å2
Dissociation area: 2,073.92 Å2
Dissociation energy (ΔGdiss): 34.69 kcal/mol
Dissociation entropy (TΔSdiss): 12.71 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-274275

Macromolecules

ATP synthase subunit c
Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9
Length: 81 amino acids
Theoretical weight: 8.06 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Mycolicibacterium smegmatis
UniProt:
  • Canonical: P9WPS1 (Residues: 1-81; Coverage: 100%)
Gene names: MTCY373.25, Rv1305, atpE
Pfam: ATP synthase subunit C
ATP synthase subunit c in PDB entry 8jr1, assembly 1, front view.
ATP synthase subunit c in PDB entry 8jr1, assembly 1, side view.
ATP synthase subunit c in PDB entry 8jr1, assembly 1, top view.
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ATP synthase subunit a
Chain: a
Length: 250 amino acids
Theoretical weight: 27.49 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Mycolicibacterium smegmatis
UniProt:
  • Canonical: P9WPV7 (Residues: 1-250; Coverage: 100%)
Gene names: MTCY373.24, Rv1304, atpB
Pfam: ATP synthase A chain
ATP synthase subunit a in PDB entry 8jr1, assembly 1, front view.
ATP synthase subunit a in PDB entry 8jr1, assembly 1, side view.
ATP synthase subunit a in PDB entry 8jr1, assembly 1, top view.
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