› 8jug

X-ray diffraction
1.3Å resolution

Function and Biology Details

Reaction catalysed: 
Cleavage of 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in B chain of insulin. No action on collagen types I, II, IV, V. Cleaves gelatin chain alpha-2(I) > alpha-1(I).
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-272476 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Matrilysin Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09237 (Residues: 95-267; Coverage: 69%)
Gene names: MMP7, MPSL1, PUMP1
Sequence domains: Matrixin
Peptide Inhibitor Chain: C
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 938 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand ZN 2 x ZN
3 modified residues:
Ligand GGL 1 x GGL
Ligand V1C 1 x V1C
Ligand TBG 1 x TBG

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I4
Unit cell:
a: 76.06Å b: 76.06Å c: 61.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.221 0.238
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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